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WNK3 antibody

Anti-WNK3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04322
Anti-WNK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX04322
Anti-WNK3 antibody used in Western Blot (WB). GTX04322
Anti-WNK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX04322
Anti-WNK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX04322
Anti-WNK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX04322

Cat. No. GTX04322

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all WNK3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

198 kDa. ( Note )

Predict Reactivity

Rabbit, Dog, Pig, Horse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human WNK3(Accession Q9BYP7), corresponding to amino acid residues C1627-L1653.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

WNK lysine deficient protein kinase 3 , PRKWNK3

Cellular Localization

Cytoplasm

Background

This gene encodes a protein belonging to the 'with no lysine' family of serine-threonine protein kinases. These family members lack the catalytic lysine in subdomain II, and instead have a conserved lysine in subdomain I. This family member functions as a positive regulator of the transcellular Ca2+ transport pathway, and it plays a role in the increase of cell survival in a caspase-3-dependent pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-WNK3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04322

GTX04322 ICC/IF Image

ICC/IF analysis of PFA fixed A549 cells using GTX04322 WNK3 antibody.
Red : Primary antibody
Green : beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100

Anti-WNK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX04322

GTX04322 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX04322 WNK3 antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-WNK3 antibody used in Western Blot (WB). GTX04322

GTX04322 WB Image

WB analysis of mouse brain tissue lysate using GTX04322 WNK3 antibody. The lane on the left was treated with the antigen-specific peptide.

Anti-WNK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX04322

GTX04322 IHC-P Image

IHC-P analysis of human ovarian cancer tissue using GTX04322 WNK3 antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-WNK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX04322

GTX04322 IHC-P Image

IHC-P analysis of rat heart tissue using GTX04322 WNK3 antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-WNK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX04322

GTX04322 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX04322 WNK3 antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 399