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WRN antibody

Anti-WRN antibody used in IHC (Paraffin sections) (IHC-P). GTX101081
Anti-WRN antibody used in Western Blot (WB). GTX101081
Anti-WRN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101081

Cat No. GTX101081

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

162 kDa. ( Note )

Positive Control

293T , A431
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human WRN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Werner syndrome RecQ like helicase,RECQ3,RECQL2,RECQL3

Cellular Localization

Nucleus , nucleolus

Background

This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-WRN antibody used in IHC (Paraffin sections) (IHC-P). GTX101081

GTX101081 IHC-P Image

WRN antibody detects WRN protein at nucleus on human normal colon by immunohistochemical analysis.
Sample: Paraffin-embedded human normal colon.
WRN antibody (GTX101081) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-WRN antibody used in Western Blot (WB). GTX101081

GTX101081 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
5% SDS PAGE
GTX101081 diluted at 1:1000

Anti-WRN antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101081

GTX101081 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using WRN(GTX101081) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169