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WTAP antibody

Orthogonal
Anti-WTAP antibody used in Western Blot (WB). GTX102708
Anti-WTAP antibody used in Western Blot (WB). GTX102708
Anti-WTAP antibody used in IHC (Paraffin sections) (IHC-P). GTX102708
Anti-WTAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102708

Cat. No. GTX102708

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all WTAP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

Jurkat nuclear extract , K562 nuclear extract

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human WTAP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038251

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

WT1 associated protein , Mum2

Cellular Localization

Nucleus , nucleolus

Background

The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in three transcript variants, two of which encode the same isoform. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-WTAP antibody used in Western Blot (WB). GTX102708

GTX102708 WB Image

Orthogonal

Jurkat whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with WTAP antibody (GTX102708) diluted at 1:500.

Anti-WTAP antibody used in Western Blot (WB). GTX102708

GTX102708 WB Image

Orthogonal

K562 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with WTAP antibody (GTX102708) diluted at 1:500.

Anti-WTAP antibody used in IHC (Paraffin sections) (IHC-P). GTX102708

GTX102708 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using WTAP(GTX102708) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-WTAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102708

GTX102708 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using WTAP(GTX102708) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169