APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:20000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
Mouse brain , Raji , rat brain
Predict Reactivity
Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human WWOX. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1952586
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
WW domain containing oxidoreductase , D16S432E , EIEE28 , FOR , FRA16D , HHCMA56 , PRO0128 , SCAR12 , SDR41C1 , WOX1
Cellular Localization
Cytoplasm , Nucleus , Mitochondrion , Golgi apparatus
Background
WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 2 WW domains and a short-chain dehydrogenase/reductase domain (SRD). The highest normal expression of this gene is detected in hormonally regulated tissues such as testis, ovary, and prostate. This expression pattern and the presence of an SRD domain suggest a role for this gene in steroid metabolism. The encoded protein is more than 90% identical to the mouse protein, which is an essential mediator of tumor necrosis factor-alpha-induced apoptosis, suggesting a similar, important role in apoptosis for the human protein. In addition, there is evidence that this gene behaves as a suppressor of tumor growth. Alternative splicing of this gene generates transcript variants that encode different isoforms. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX113325 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed A549, using WWOX(GTX113325) antibody at 1:200 dilution.
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GTX113325 WB Image
Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with WWOX antibody (GTX113325) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113325 IHC-P Image
WWOX antibody detects WWOX protein at cytoplasm on human placenta by immunohistochemical analysis.
Sample: Paraffin-embedded placenta.
WWOX antibody (GTX113325) dilution: 1:100.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX113325 IHC-P Image
WWOX antibody detects WWOX protein at cytosol on mouse skin by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
WWOX antibody (GTX113325) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX113325 WB Image
Sample (30 μg of whole cell lysate)
A: Raji
12% SDS PAGE
GTX113325 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113325 WB Image
Sample (50 μg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX113325 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113325 IP Image
WWOX antibody immunoprecipitates WWOX protein in IP experiments.
IP samples: 293T whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of WWOX protein by 4 μg WWOX antibody (GTX113325)
10 % SDS-PAGE
The immunoprecipitated WWOX protein was detected by WWOX antibody (GTX113325) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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REFERENCE
There are currently no references for WWOX antibody (GTX113325). Be the first to share your publications with this product.
REVIEW
WWOX antibody
Cat. No. GTX113325
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Rating
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( Average 4 based on 1 users reviews)
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Application
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Western Blot(WB)
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( Average 4 based on 1 users reviews)
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Date :
Anonymous submitted on 13-May-2015
Application Tested :
WB
Sample Species :
Hu
Sample :
HepG2 cells
Amount used :
20μg
Blocking :
5% milk in PBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
