West Nile virus Envelope antibody [GT3029]

Cat. No. GTX633919

Host	Mouse
Clonality	Monoclonal
Clone Name	GT3029
Isotype	IgG1
Application	WB, ICC/IF
Reactivity	West Nile virus
Package	100 μl ($399), 25 μl ($169)

See all West Nile virus Envelope products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

West Nile virus Envelope protein E-transfected 293T

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

West Nile virus

Immunogen

Recombinant protein encompassing a sequence within the center region of West Nile virus Envelope protein (West Nile virus (strain NY99-IC)). The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888391

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

West Nile virus Envelope protein , WNV Envelope protein

Research Area

DATA IMAGES

GTX633919 ICC/IF Image

West Nile virus Envelope protein antibody [GT3029] detects West Nile virus Envelope protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: West Nile virus Envelope protein stained by West Nile virus Envelope protein antibody [GT3029] (GTX633919) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

GTX633919 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with West Nile virus Envelope protein antibody [GT3029] (GTX633919) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations

West Nile virus Envelope antibody [GT3029]

Cat. No. GTX633919

Host

Clonality

Clone Name

Isotype

Application

Reactivity