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West Nile virus Envelope antibody

Anti-West Nile virus Envelope antibody used in Western Blot (WB). GTX132052
Anti-West Nile virus Envelope antibody used in Western Blot (WB). GTX132052
Anti-West Nile virus Envelope antibody used in Western Blot (WB). GTX132052

Cat No. GTX132052

Reference 1
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 West Nile virus
Package
100 μl ($379),
25 μl ($169)
See all West Nile virus Envelope products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

WNV-infected 293T , WNV-infected vero cell , WNV Envelope-transfected 293T cells

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

West Nile virus

Immunogen

Recombinant protein encompassing a sequence within the center region of West Nile virus Envelope protein (West Nile virus (strain NY99-IC)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886550

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

West Nile virus Envelope protein , WNV Envelope protein

Research Area

DATA IMAGES

Anti-West Nile virus Envelope antibody used in Western Blot (WB). GTX132052

GTX132052 WB Image

Non-infected (–) and infected (+) 293T whole cell extracts were separated by 4-20% SDS-PAGE, and the membrane was blotted with West Nile virus Envelope protein antibody (GTX132052) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-West Nile virus Envelope antibody used in Western Blot (WB). GTX132052

GTX132052 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with West Nile virus Envelope protein antibody (GTX132052) diluted at 1:5000.

Anti-West Nile virus Envelope antibody used in Western Blot (WB). GTX132052

GTX132052 WB Image

Non-infected (–) and infected Vero whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with West Nile virus Envelope protein antibody (GTX132052).

REFERENCE

Application Reference

Akhras S et al. Viruses 2019; 11 (8) ZIKV Envelope Domain-Specific Antibodies: Production, Purification and Characterization.
Application : WB
Submit a Reference

REVIEW

West Nile virus Envelope antibody Cat No. GTX132052

Rating
( Average 4.5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 4.5 based on 2 users reviews)
Anti-West Nile virus Envelope antibody used in Western Blot (WB). GTX132052
Date : Anonymous submitted on 15-May-2018
Score :
Application Tested : WB
Sample Species : WNV
Primary Antibody : 1:2000, 25°C, 1Hr
Lane Description : Non-infected (–) and infected (+) 293T whole cell extracts.
Figure Note : Image courtesy of Alanna Tseng. (Laboratory of Dr. Pakieli Kaufusi, University of Hawaii at Manoa, JABSOM)
Anti-West Nile virus Envelope antibody used in Western Blot (WB). GTX132052
Date : Anonymous submitted on 06-Apr-2016
Score :
Application Tested : WB
Sample Species : WNV
Primary Antibody : 1:5000, 4°C, 16Hr
Lane Description : Lane 1: WNV-NY99 infected vero cells, Lane 2: DTV infected vero cells, Lane 3: JEV infected vero cells, Lane 4: vero cells
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169