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West Nile virus NS2B protein antibody

Anti-West Nile virus NS2B protein antibody used in Western Blot (WB). GTX132060
Anti-West Nile virus NS2B protein antibody used in Western Blot (WB). GTX132060
Anti-West Nile virus NS2B protein antibody used in Western Blot (WB). GTX132060

Cat No. GTX132060

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

West Nile virus
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Positive Control

WNV-infected 293T , WNV-infected vero cell , JEV-infected vero cell , WNV NS2B-transfected 293T cells

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.58 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

West Nile virus

Immunogen

Full length West Nile virus NS2B recombinant protein. (West Nile virus (strain NY99-IC))

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886552

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

West Nile virus Non-structural protein 2B , West Nile virus NS2B , WNV Non-structural protein 2B , WNV NS2B

Research Area

DATA IMAGES

Anti-West Nile virus NS2B protein antibody used in Western Blot (WB). GTX132060

GTX132060 WB Image

Non-infected (–) and infected (+) 293T whole cell extracts were separated by 4-20% SDS-PAGE, and the membrane was blotted with West Nile virus NS2B protein antibody (GTX132060) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-West Nile virus NS2B protein antibody used in Western Blot (WB). GTX132060

GTX132060 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with West Nile virus NS2B protein antibody (GTX132060) diluted at 1:5000.

Anti-West Nile virus NS2B protein antibody used in Western Blot (WB). GTX132060

GTX132060 WB Image

Non-infected (–) and infected Vero whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with West Nile virus NS2B protein antibody (GTX132060).

REFERENCE

REVIEW

West Nile virus NS2B protein antibody Cat No. GTX132060

Rating ( Average 4 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4 based on 2 users reviews)
Date : Anonymous submitted on 15-May-2018
Score :
Application Tested : WB
Sample Species : WNV
Primary Antibody : 1:1500, 25°C, 1Hr
Lane Description : Non-infected (–) and infected (+) 293T whole cell extracts.
Figure Note : Image courtesy of Alanna Tseng. (Laboratory of Dr. Pakieli Kaufusi, University of Hawaii at Manoa, JABSOM)
Date : Anonymous submitted on 06-Apr-2016
Score :
Application Tested : WB
Sample Species : WNV
Primary Antibody : 1:5000, 4°C, 16Hr
Lane Description : Lane 1: WNV-NY99 infected vero cells, Lane 2: DTV infected vero cells, Lane 3: JEV infected vero cells, Lane 4: vero cells
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169