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West Nile virus NS2B protein antibody

Anti-West Nile virus NS2B protein antibody used in Western Blot (WB). GTX132060
Anti-West Nile virus NS2B protein antibody used in Western Blot (WB). GTX132060
Anti-West Nile virus NS2B protein antibody used in Western Blot (WB). GTX132060

Cat No. GTX132060

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

West Nile virus
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Positive Control

WNV-infected 293T , WNV-infected vero cell , JEV-infected vero cell , WNV NS2B-transfected 293T cells

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.58 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Full length Powassan virus NS2B recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

West Nile virus Non-structural protein 2B , West Nile virus NS2B , WNV Non-structural protein 2B , WNV NS2B

Research Area

DATA IMAGES

Anti-West Nile virus NS2B protein antibody used in Western Blot (WB). GTX132060

GTX132060 WB Image

Non-infected (–) and infected (+) 293T whole cell extracts were separated by 4-20% SDS-PAGE, and the membrane was blotted with West Nile virus NS2B protein antibody (GTX132060) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-West Nile virus NS2B protein antibody used in Western Blot (WB). GTX132060

GTX132060 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with West Nile virus NS2B protein antibody (GTX132060) diluted at 1:5000.

Anti-West Nile virus NS2B protein antibody used in Western Blot (WB). GTX132060

GTX132060 WB Image

Non-infected (–) and infected Vero whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with West Nile virus NS2B protein antibody (GTX132060).

REFERENCE

There are currently no references for West Nile virus NS2B protein antibody (GTX132060). Be the first to share your publications with this product.

REVIEW

West Nile virus NS2B protein antibody Cat No. GTX132060

Rating ( Average 4 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4 based on 2 users reviews)
Date : Anonymous submitted on 15-May-2018
Score :
Application Tested : WB
Sample Species : WNV
Primary Antibody : 1:1500, 25°C, 1Hr
Lane Description : Non-infected (–) and infected (+) 293T whole cell extracts.
Figure Note : Image courtesy of Alanna Tseng. (Laboratory of Dr. Pakieli Kaufusi, University of Hawaii at Manoa, JABSOM)
Date : Anonymous submitted on 06-Apr-2016
Score :
Application Tested : WB
Sample Species : WNV
Primary Antibody : 1:5000, 4°C, 16Hr
Lane Description : Lane 1: WNV-NY99 infected vero cells, Lane 2: DTV infected vero cells, Lane 3: JEV infected vero cells, Lane 4: vero cells
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf