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West Nile virus NS3 protein antibody

Anti-West Nile virus NS3 protein antibody used in Western Blot (WB). GTX131955
Anti-West Nile virus NS3 protein antibody used in Western Blot (WB). GTX131955
Anti-West Nile virus NS3 protein antibody used in Western Blot (WB). GTX131955

Cat. No. GTX131955

Reference 2
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 West Nile virus
Package
100 μl ($399),
25 μl ($169)
See all West Nile virus NS3 protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Positive Control

WNV-infected vero cell , WNV NS3-transfected 293T cells , WNV-infected 293T

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.94 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

West Nile virus

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of West Nile virus NS3 protein (West Nile virus (strain NY99-IC)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886542

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

West Nile virus Non-structural protein 3 , West Nile virus NS3 , WNV Non-structural protein 3 , WNV NS3

Research Area

DATA IMAGES

Anti-West Nile virus NS3 protein antibody used in Western Blot (WB). GTX131955

GTX131955 WB Image

Non-infected (–) and infected (+) 293T whole cell extracts were separated by 4-20% SDS-PAGE, and the membrane was blotted with West Nile virus NS3 protein antibody (GTX131955) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-West Nile virus NS3 protein antibody used in Western Blot (WB). GTX131955

GTX131955 WB Image

Non-infected (–) and infected Vero whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with West Nile virus NS3 protein antibody (GTX131955).

Anti-West Nile virus NS3 protein antibody used in Western Blot (WB). GTX131955

GTX131955 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with West Nile virus NS3 protein antibody (GTX131955) diluted at 1:5000.

REFERENCE

Application Reference

Tseng AC et al. Viruses 2021; 13 (2) Potential Dual Role of West Nile Virus NS2B in Orchestrating NS3 Enzymatic Activity in Viral Replication.
Application : WB, ICC/IF
Boys IN et al. Cell Host Microbe 2020; 28 (5) : 712-723.e9 RTP4 Is a Potent IFN-Inducible Anti-flavivirus Effector Engaged in a Host-Virus Arms Race in Bats and Other Mammals.
Application : WB
Submit a Reference

REVIEW

West Nile virus NS3 protein antibody Cat. No. GTX131955

Rating
( Average 3.5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 3.5 based on 2 users reviews)
Anti-West Nile virus NS3 protein antibody used in Western Blot (WB). GTX131955
Date : Anonymous submitted on 15-May-2018
Score :
Application Tested : WB
Sample Species : WNV
Primary Antibody : 1:1500, 25°C, 1Hr
Lane Description : Non-infected (–) and infected (+) 293T whole cell extracts.
Figure Note : Image courtesy of Alanna Tseng. (Laboratory of Dr. Pakieli Kaufusi, University of Hawaii at Manoa, JABSOM)
Anti-West Nile virus NS3 protein antibody used in Western Blot (WB). GTX131955
Date : Anonymous submitted on 06-Apr-2016
Score :
Application Tested : WB
Sample Species : WNV
Primary Antibody : 1:5000, 4°C, 16Hr
Lane Description : Lane 1: WNV-NY99 infected vero cells, Lane 2: DTV infected vero cells, Lane 3: JEV infected vero cells, Lane 4: vero cells
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169