GeneTex
United States (US)

West Nile virus NS3 protein antibody

GTX131955_42151_20180525_WB_WNV_18121410_489.jpg
GTX131955_42151_20160602_WB_B_18121410_260.jpg
GTX131955_42151_20160317_WB_WNV_DTV_JEV_18121410_920.jpg

Cat No. GTX131955

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity West Nile virus
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Positive Control

WNV-infected 293T , WNV-infected vero cell , JEV-infected vero cell , WNV NS3-transfected 293T cells
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.94 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of West Nile virus NS3 protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

WestNile virus (WNV) NS3 antibody, WestNile virus NS3 protein antibody, WNV NS3 antibody

Research Area

DATA IMAGES
GTX131955_42151_20180525_WB_WNV_18121410_489.jpg

GTX131955 WB Image

Non-infected (–) and infected (+) 293T whole cell extracts were separated by 4-20% SDS-PAGE, and the membrane was blotted with West Nile virus NS3 protein antibody (GTX131955) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX131955_42151_20160602_WB_B_18121410_260.jpg

GTX131955 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with West Nile virus NS3 protein antibody (GTX131955) diluted at 1:5000.

GTX131955_42151_20160317_WB_WNV_DTV_JEV_18121410_920.jpg

GTX131955 WB Image

Non-infected (–) and infected Vero whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with West Nile virus NS3 protein antibody (GTX131955).

REVIEW

West Nile virus NS3 protein antibody Cat No. GTX131955

Rating ( Average 3.5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 3.5 based on 2 users reviews)
Date : Anonymous submitted on 15-May-2018
Score :
Application Tested : WB
Sample Species : WNV
Primary Antibody : 1:1500, 25°C, 1Hr
Lane Description : Non-infected (–) and infected (+) 293T whole cell extracts. Image courtesy of Alanna Tseng. (Laboratory of Dr. Pakieli Kaufusi, University of Hawaii at Manoa, JABSOM)
Date : Anonymous submitted on 06-Apr-2016
Score :
Application Tested : WB
Sample Species : WNV
Primary Antibody : 1:5000, 4°C, overnightHr
Lane Description : Lane 1: WNV-NY99 infected vero cells, Lane 2: DTV infected vero cells, Lane 3: JEV infected vero cells, Lane 4: vero cells
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169