GeneTex
United States (US)

Wilms Tumor 1 antibody [6f-H17]

Cat No. GTX30735

Host Mouse
Clonality Monoclonal
Clone Name 6f-H17
Isotype IgG1
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

For IF: Use at a dilution of 1:50-1:500. For IHC-P: Use at a dilution of 1:50-1:500. For IP: Use 1-2μg for 100μg of total protein - 1ml cell lysate. For WB: Use at a dilution of 1:200-1:500. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

49 kDa. ( Note )

Positive Control

WB: K562 whole cell lysate and MOLT-4 whole cell lysate
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.2, containing 0.2% BSA and 0.09% sodiium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant Wilms tumor 1 human protein

Purification

Protein A affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Wilms Tumor 1,Awt1,Gud,Nphs4,Wagr,Wit-2,Wt33,Wt1

Background

WT1 encodes multiple isoforms of a zinc finger transcription factor essential for correct mammalian urogenital development. Different WT1 isoforms are thought to regulate transcription and participate in mRNA processing, functions reflected by a complex sub-nuclear distribution. In humans, the preservation of the correct isoform ratio and their localization has been reported to be vital, and its disruption to result in disease. This gene has been reported as a useful marker for several types of illnesses. Mutations in this gene lead to the development of Wilm’s Tumor, a sporadic and familial childhood kidney tumor.

Database

Research Area

Package List Price ($)
$ 319