APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml for 30 minutes at RT |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
Product Note
Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm's tumors.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein corresponding to residues 1-181 of human WT1.
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
AWT1 , EWSWT1 , GUD , NPHS4 , WAGR , WIT2 , WT1 , WT33 , Wilms tumor 1 , Wilms Tumor 1 , WT1 transcription factor
Cellular Localization
Nucleus,Nucleolus,Cytoplasm,Nucleus speckle,Nucleoplasm
Background
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015]
Database
Research Area
DATA IMAGES
![Anti-Wilms Tumor 1 antibody [SPM361] used in IHC (Paraffin sections) (IHC-P). GTX35173](/templates/default/js/lightbox/images/loading.gif "Anti-Wilms Tumor 1 antibody [SPM361] used in IHC (Paraffin sections) (IHC-P). GTX35173")
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GTX35173 IHC-P Image
IHC-P analysis of human mesothelioma tissue using GTX35173 Wilms Tumor 1 antibody [SPM361].
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REFERENCE
There are currently no references for Wilms Tumor 1 antibody [SPM361] (GTX35173). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Wilms Tumor 1 antibody [SPM361] (GTX35173). Be the first to share your experience with this product.
![Anti-Wilms Tumor 1 antibody [SPM361] used in IHC (Paraffin sections) (IHC-P). GTX35173](/upload/website/prouct_img/small/GTX35173/GTX35173_20200115_IHC-P_797_w_23060819_261.webp "Anti-Wilms Tumor 1 antibody [SPM361] used in IHC (Paraffin sections) (IHC-P). GTX35173")
![Anti-Wilms Tumor 1 antibody [SPM361] used in IHC (Paraffin sections) (IHC-P). GTX35173](/upload/website/prouct_img/normal/GTX35173/GTX35173_20200115_IHC-P_797_w_23060819_261.webp "Anti-Wilms Tumor 1 antibody [SPM361] used in IHC (Paraffin sections) (IHC-P). GTX35173")