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Wilms Tumor 1 antibody [WT1/1434R]

Anti-Wilms Tumor 1 antibody [WT1/1434R] used in IHC (Paraffin sections) (IHC-P). GTX02743
Anti-Wilms Tumor 1 antibody [WT1/1434R] used in IHC (Paraffin sections) (IHC-P). GTX02743

Cat No. GTX02743

Host

Rabbit

Clonality

Monoclonal

Clone Name

WT1/1434R

Isotype

IgG

Application

IHC-P

Reactivity

Human, Rat
Package
100 μg ($429)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P 1-2 μg/ml

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

200 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human full-length WT1 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

WT1 transcription factor , AWT1 , GUD , NPHS4 , WAGR , WIT-2 , WT33

Cellular Localization

Nucleus , Cytoplasm , Nucleolus

Background

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015]

Database

Research Area

DATA IMAGES

Anti-Wilms Tumor 1 antibody [WT1/1434R] used in IHC (Paraffin sections) (IHC-P). GTX02743

GTX02743 IHC-P Image

IHC-P analysis of human mesothelioma section using GTX02743 Wilms Tumor 1 antibody [WT1/1434R].

Anti-Wilms Tumor 1 antibody [WT1/1434R] used in IHC (Paraffin sections) (IHC-P). GTX02743

GTX02743 IHC-P Image

IHC-P analysis of rat testis tissue section using GTX02743 Wilms Tumor 1 antibody [WT1/1434R].

REFERENCE

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REVIEW

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