GeneTex
United States (US)

Wilms Tumor 1 antibody [WT1/857]

GTX35174_20170712_IHC-P_1_18121410_749.jpg
GTX35174_20170712_IHC-P_2_18121410_933.jpg
GTX35174_20170712_IHC-P_18121410_733.jpg

Cat No. GTX35174

Host Mouse
Clonality Monoclonal
Clone Name WT1/857
Isotype IgG1
Application ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 0.5-1ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Specificity/Sensitivity

Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm s tumors.ĀWT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm s tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.
PROPERTIES

Form

Liquid

Buffer

Prepared in 1mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human WT1 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Wilms Tumor 1,Awt1,Gud,Nphs4,Wagr,Wit-2,Wt33,Wt1

Background

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Oct 2010]

Database

Research Area

DATA IMAGES
GTX35174_20170712_IHC-P_1_18121410_749.jpg

GTX35174 IHC-P Image

Formalin-fixed, paraffin-embedded Mouse Kidney stained with WT1 Monoclonal Antibody (WT1/857).

GTX35174_20170712_IHC-P_2_18121410_933.jpg

GTX35174 IHC-P Image

Formalin-fixed, paraffin-embedded Rat kidney stained with WT1 Monoclonal Antibody (WT1/857).

GTX35174_20170712_IHC-P_18121410_733.jpg

GTX35174 IHC-P Image

Formalin-fixed, paraffin-embedded human Fetal Kidney stained with WT1 Monoclonal Antibody (WT1/857).

Package List Price ($)
$ 329