GeneTex

  • Country / Location Selection

United States (US)

Wilms Tumor 1 antibody [WT49]

Anti-Wilms Tumor 1 antibody [WT49] used in IHC (Paraffin sections) (IHC-P). GTX01958

Cat. No. GTX01958

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 WT49

Isotype

 IgG1

Application

 IHC-P

Reactivity

 Human
Package
500 μl ($659)
See all Wilms Tumor 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100

Note :

IHC-P
The recommended fixative is 10% neutral-buffered formalin for paraffin-embedded tissue sections. Heat Induced Epitope Retreival (HIER) was performed in Epitope Retrieval Solution pH 9.
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tissue culture supernatant

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Antigen Species

Human

Immunogen

A prokaryotic recombinant protein containing 1–181 amino acids of the N-terminal of the Wilms’ Tumor protein.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

WT1 transcription factor , AWT1 , GUD , NPHS4 , WAGR , WIT-2 , WT33

Cellular Localization

Nucleus , Cytoplasm , Nucleolus

Background

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015]

Database

Research Area

DATA IMAGES

Anti-Wilms Tumor 1 antibody [WT49] used in IHC (Paraffin sections) (IHC-P). GTX01958

GTX01958 IHC-P Image

IHC-P analysis of human kidney tissue using GTX01958 Wilms Tumor 1 antibody [WT49]. Note the nuclear staining of invasive tumor cells.

REFERENCE

Application Reference

A Iliesiu et al. Oncology Letters 2023; 26 : 442 A pancreatic adenocarcinoma mimicking hepatoid carcinoma of uncertain histogenesis: A case report and literature review
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for Wilms Tumor 1 antibody [WT49] (GTX01958). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 659