APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Mouse testis,Rat testis
Predict Reactivity
Human, Chicken, Pig, Japanese Medaka, Xenopus laevis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.85mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Wilms Tumor 1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
AWT1 antibody, GUD antibody, NPHS4 antibody, WAGR antibody, WIT-2 antibody, WT33 antibody, WT1 antibody, Wilms tumor protein antibody, amino-terminal domain of EWS,last three zinc fingers of the DNA-binding domain of WT1 antibody, Wilms tumor 1 antibody
Cellular Localization
Nucleus
Background
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX100563 IHC-P Image
Wilms Tumor 1 antibody detects Wilms Tumor 1 protein at cytoplasm in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Wilms Tumor 1 antibody (GTX100563) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX100563 IHC-P Image
Wilms Tumor 1 antibody detects WT1 protein at cytoplasm in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Wilms Tumor 1 antibody (GTX100563) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX100563 IHC-P Image
Wilms Tumor 1 antibody detects Wilms Tumor 1 protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Wilms Tumor 1 antibody (GTX100563) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX100563 IHC-P Image
Wilms Tumor 1 antibody detects WT1 protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Wilms Tumor 1 antibody (GTX100563) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
REFERENCE
