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Wilms Tumor 1 antibody

Anti-Wilms Tumor 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131203

Cat. No. GTX131203

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Wilms Tumor 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.28 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Wilms Tumor 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886442

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

WT1 transcription factor , AWT1 , GUD , NPHS4 , WAGR , WIT-2 , WT33

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Wilms Tumor 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131203

GTX131203 ICC/IF Image

Wilms Tumor 1 antibody detects Wilms Tumor 1 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Wilms Tumor 1 stained by Wilms Tumor 1 antibody (GTX131203) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169