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Wilms Tumor 1 antibody

Anti-Wilms Tumor 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32972
Anti-Wilms Tumor 1 antibody used in Western Blot (WB). GTX32972
Anti-Wilms Tumor 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32972

Cat No. GTX32972

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-302 of human WT1 (NP_001185480.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

WT1 transcription factor , AWT1 , GUD , NPHS4 , WAGR , WIT-2 , WT33

Cellular Localization

Nucleus,Cytoplasm,Nucleolus

Background

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015]

Database

Research Area

DATA IMAGES

Anti-Wilms Tumor 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32972

GTX32972 IHC-P Image

IHC-P analysis of human liver injury tissue using GTX32972 Wilms Tumor 1 antibody.
Dilution : 1:100

Anti-Wilms Tumor 1 antibody used in Western Blot (WB). GTX32972

GTX32972 WB Image

WB analysis of various samples using GTX32972 Wilms Tumor 1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Wilms Tumor 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32972

GTX32972 ICC/IF Image

ICC/IF analysis of HeLa cell using GTX32972 Wilms Tumor 1 antibody.
Blue : DAPI

REFERENCE

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REVIEW

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Package List Price ($)
$ 329