APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 - 2 μg/mL |
| 5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Human Lung Tissue Slide , 3T3 Cell Lysate
Product Note
At least two isoforms are known to exist; this antibody will only detect the larger isoform.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Wip1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human Wip1.The immunogen is located within the last 50 amino acids of Wip1.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein phosphatase, Mg2+/Mn2+ dependent 1D , IDDGIP , PP2C-DELTA , WIP1
Cellular Localization
Nucleus,Cytoplasm, cytosol
Background
The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX31604 IHC-P Image
IHC-P analysis of human lung carcinoma tissue using GTX31604 Wip1 antibody.
Working concentration : 20 μg/ml
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GTX31604 IHC-P Image
IHC-P analysis of human lung carcinoma tissue using GTX31604 Wip1 antibody.
Working concentration : 5 μg/ml
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GTX31604 WB Image
WB analysis of 3T3 cell lysate using GTX31604 Wip1 antibody.
Working concentration : 1 μg/ml
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REFERENCE
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REVIEW
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