GeneTex
United States (US)

Wip1 antibody

Anti-Wip1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31604
Anti-Wip1 antibody used in Western Blot (WB). GTX31604
Anti-Wip1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31604

Cat No. GTX31604

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

Positive Control

Human Lung Tissue Slide , 3T3 Cell Lysate

Specificity/Sensitivity

At least two isoforms are known to exist; this antibody will only detect the larger isoform.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Wip1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human Wip1.The immunogen is located within the last 50 amino acids of Wip1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein Phosphatase, Mg2+/Mn2+ Dependent 1D,Iddgip,Pp2C-Delta,Wip1,Ppm1D

Background

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Wip1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31604

GTX31604 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX31604 Wip1 antibody.
Working concentration : 5 μg/ml

Anti-Wip1 antibody used in Western Blot (WB). GTX31604

GTX31604 WB Image

WB analysis of 3T3 cell lysate using GTX31604 Wip1 antibody.
Working concentration : 1 μg/ml

Anti-Wip1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31604

GTX31604 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX31604 Wip1 antibody.
Working concentration : 20 μg/ml

Package List Price ($)
$ 339