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Wnt1 antibody, C-term

Anti-Wnt1 antibody, C-term used in Western Blot (WB). GTX81721
Anti-Wnt1 antibody, C-term used in Flow cytometry (FACS). GTX81721
Anti-Wnt1 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX81721

Cat. No. GTX81721

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human, Mouse
Package
400 μl ($399)
See all Wnt1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:10-1:50
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 260-288 amino acids from the C-terminal region of human WNT1.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Wnt family member 1 , BMND16 , INT1 , OI15

Cellular Localization

Secreted

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Wnt1 antibody, C-term used in Western Blot (WB). GTX81721

GTX81721 WB Image

WB analysis of mouse heart tissue lysate (35ug/lane) using GTX81721 Wnt1 antibody, C-term.

Anti-Wnt1 antibody, C-term used in Flow cytometry (FACS). GTX81721

GTX81721 FACS Image

FACS analysis of MCF-7 cells using GTX81721 Wnt1 antibody, C-term.
Green : primary antibody
Blue : negative control

Anti-Wnt1 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX81721

GTX81721 IHC-P Image

IHC-P analysis of human testis tissue using GTX81721 Wnt1 antibody, C-term.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399