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Wnt10b antibody [5A7]

Anti-Wnt10b antibody [5A7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83307
Anti-Wnt10b antibody [5A7] used in IHC (Paraffin sections) (IHC-P). GTX83307
Anti-Wnt10b antibody [5A7] used in Western Blot (WB). GTX83307

Cat. No. GTX83307

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5A7

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human
Package
100 μl ($409)
See all Wnt10b products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human WNT10B expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_11166312

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Wnt family member 10B , SHFM6 , STHAG8 , WNT-12

Cellular Localization

Secreted

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level. This gene is clustered with another family member, WNT1, in the chromosome 12q13 region. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Wnt10b antibody [5A7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83307

GTX83307 ICC/IF Image

ICC/IF analysis of PANC-1 cells using GTX83307 WNT10B antibody [5A7].
Green : WNT10B
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-Wnt10b antibody [5A7] used in IHC (Paraffin sections) (IHC-P). GTX83307

GTX83307 IHC-P Image

IHC-P analysis of human normal stomach (A), normal liver (B), normal kidney (C) and rectum cancer tissue (D) using GTX83307 WNT10B antibody [5A7].

Anti-Wnt10b antibody [5A7] used in Western Blot (WB). GTX83307

GTX83307 WB Image

WB analysis of HeLa cell lysate using GTX83307 WNT10B antibody [5A7].

REFERENCE

Application Reference

Lee JG et al. J Biol Chem 2015; 290 (44) : 26752-64 WNT10B enhances proliferation through β-catenin and RAC1 GTPase in human corneal endothelial cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 409