Wnt10b antibody [HL2370]

Wnt10b antibody [HL2370] detects Wnt10b protein at cell membrane by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Wnt10b stained by Wnt10b antibody [HL2370] (GTX638576) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt10b antibody [HL2370] (GTX638576) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt10b antibody [HL2370] (GTX638576) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Wnt10b antibody [HL2370] detects Wnt10b protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Wnt10b stained by Wnt10b antibody [HL2370] (GTX638576) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Wnt10b antibody [HL2370] detects Wnt10b protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
Wnt10b stained by Wnt10b antibody [HL2370] (GTX638576) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt10b antibody [HL2370] (GTX638576) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.