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Wnt10b antibody

Anti-Wnt10b antibody used in IHC (Paraffin sections) (IHC-P). GTX17006
Anti-Wnt10b antibody used in Western Blot (WB). GTX17006

Cat. No. GTX17006

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all Wnt10b products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 - 4 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

Human Skeletal Muscle Tissue Lysate

Product Note

Two isoforms of Wnt10b are known to exist; this antibody will only recognize the longer isoform. This Wnt10b antibody will not cross-react with Wnt10a.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Wnt10b antibody was raised against a 15 amino acid synthetic peptide from near the center of human Wnt10b.The immunogen is located within amino acids 130 - 180 of Wnt10b.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887551

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Wnt family member 10B , SHFM6 , STHAG8 , WNT-12

Cellular Localization

Secreted

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level. This gene is clustered with another family member, WNT1, in the chromosome 12q13 region. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Wnt10b antibody used in IHC (Paraffin sections) (IHC-P). GTX17006

GTX17006 IHC-P Image

IHC-P analysis of human skeletal muscle tissue using GTX17006 WNT10B antibody.
Working concentration : 2.5 μg/ml

Anti-Wnt10b antibody used in Western Blot (WB). GTX17006

GTX17006 WB Image

WB analysis of human skeletal muscle tissue lysate using GTX17006 WNT10B antibody.
Working concentration : (A) 2 and (B) 4 μg/ml

REFERENCE

Application Reference

Shen HH et al. J Biomed Sci 2019; 26 (1) : 62 Raloxifene inhibits adipose tissue inflammation and adipogenesis through Wnt regulation in ovariectomized rats and 3?3-L1 cells.
Application : WB
Reactivity : Rat
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399