Wnt16 antibody [HL1498]

Cat. No. GTX636972

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1498
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all Wnt16 products

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Product Note

This antibody is specific for human Wnt16 protein, and it does not cross react with human Wnt1, Wnt2, Wnt2b, Wnt3, Wnt5a, Wnt7a, Wnt7b, Wnt8a, Wnt8b, Wnt9a, Wnt9b, Wnt10a, and Wnt10b protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Wnt16. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Wnt family member 16

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It contains two transcript variants diverging at the 5' termini. These two variants are proposed to be the products of separate promoters and not to be splice variants from a single promoter. They are differentially expressed in normal tissues, one of which (variant 2) is expressed at significant levels only in the pancreas, whereas another one (variant 1) is expressed more ubiquitously with highest levels in adult kidney, placenta, brain, heart, and spleen. [provided by RefSeq, Jul 2008]

Database

Gene ID: 51384 WNT16
UniProt: Q9UBV4 WNT16

Research Area

DATA IMAGES

GTX636972 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt16 antibody [HL1498] (GTX636972) diluted at 1:5000. The HRP-conjugated anti-FALSE was used to detect the primary antibody.

GTX636972 IHC-P Image

Wnt16 antibody [HL1498] detects secreted Wnt16 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Wnt16 stained by Wnt16 antibody [HL1498] (GTX636972) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX636972 ICC/IF Image

Wnt16 antibody [HL1498] detects Wnt16 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Wnt16 stained by Wnt16 antibody [HL1498] (GTX636972) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations