HepG2 whole cell extract and conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with WNT2B antibody (GTX128099) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.