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Wnt3 antibody, Internal

Anti-Wnt3 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89319
Anti-Wnt3 antibody, Internal used in Western Blot (WB). GTX89319

Cat. No. GTX89319

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all Wnt3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-3μg/ml
ICC/IF Assay dependent
IHC-P 2-4μg/ml

Note :

IHC-P
Human Breast shows cytoplasm staining in some luminal epithelial cells.
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89319-PEP).

Predict Reactivity

Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence CGRGHNTRTEKRKEK, from the internal region of the protein sequence according to NP_110380.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Wnt family member 3 , INT4 , TETAMS

Cellular Localization

Secreted

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98% amino acid identity to mouse Wnt3 protein, and 84% to human WNT3A protein, another WNT gene product. The mouse studies show the requirement of Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggest that this gene may play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the WNT-beta-catenin-TCF signaling pathway. This gene is clustered with WNT15, another family member, in the chromosome 17q21 region. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Wnt3 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89319

GTX89319 IHC-P Image

IHC-P analysis of human breast using GTX89319 WNT3 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2μg/ml

Anti-Wnt3 antibody, Internal used in Western Blot (WB). GTX89319

GTX89319 WB Image

WB analysis of HEK293 cell lysate using GTX89319 WNT3 antibody, Internal.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

Application Reference

Yin L et al. Aging (Albany NY) 2020; 12 (9) : 8261-8288 Identification of candidate lncRNAs and circRNAs regulating WNT3/β-catenin signaling in essential hypertension.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399