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Wnt3 antibody

Anti-Wnt3 antibody used in Western Blot (WB). GTX128100

Cat No. GTX128100

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

Wnt3-transfected 293T cells , Want3A-transfected 293T cells

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human WNT3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Wnt family member 3 , INT4 , TETAMS

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98% amino acid identity to mouse Wnt3 protein, and 84% to human WNT3A protein, another WNT gene product. The mouse studies show the requirement of Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggest that this gene may play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the WNT-beta-catenin-TCF signaling pathway. This gene is clustered with WNT15, another family member, in the chromosome 17q21 region. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Wnt3 antibody used in Western Blot (WB). GTX128100

GTX128100 WB Image

WNT3 antibody detects WNT3 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of Wnt3-transfected 293T cells
10 % SDS-PAGE
WNT3 antibody (GTX128100) dilution: 1:5000

REFERENCE

There are currently no references for Wnt3 antibody (GTX128100). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169