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Wnt3a antibody [3A6]

Anti-Wnt3a antibody [3A6] used in IHC (Paraffin sections) (IHC-P). GTX50033
Anti-Wnt3a antibody [3A6] used in Western Blot (WB). GTX50033

Cat No. GTX50033

Host

Mouse

Clonality

Monoclonal

Clone Name

3A6

Isotype

IgG2a

Application

WB, IHC-P, ELISA

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:1,000~1:2,000
Immunohistochemistry: Use at 1:50~100.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

39 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Wnt3a (19-352aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Wnt Family Member 3A , Wnt3A

Background

Wingless-type MMTV integration site family, member 3A (Wnt3a) is a member of the Wnt family. The Wnt gene family is representative of a growing class of multifunctioanl factors that play a role in both tumorigenesis and patterning events required for normal differentiation and development. Wnt3a is a ligand for members of the frizzled family of seven transmembrane receptors.

Database

Research Area

DATA IMAGES

Anti-Wnt3a antibody [3A6] used in IHC (Paraffin sections) (IHC-P). GTX50033

GTX50033 IHC-P Image

Paraffin embedded sections of human breast cancer tissue were incubated with anti-human Wnt3a (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Anti-Wnt3a antibody [3A6] used in Western Blot (WB). GTX50033

GTX50033 WB Image

The lysates of A549 (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Wnt3a (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

REFERENCE

There are currently no references for Wnt3a antibody [3A6] (GTX50033). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Wnt3a antibody [3A6] (GTX50033). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf