Wnt3a antibody [HL1911]

Wnt3a antibody [HL1911] detects Wnt3a protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.
Green: Wnt3a stained by Wnt3a antibody [HL1911] (GTX637660) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Wnt3a antibody [HL1911] detects Wnt3a protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
Wnt3a stained by Wnt3a antibody [HL1911] (GTX637660) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Wnt3a antibody [HL1911] detects secreted Wnt3a protein by immunohistochemical analysis.
Sample: Paraffin-embedded human prostate cancer.
Wnt3a stained by Wnt3a antibody [HL1911] (GTX637660) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt3a antibody [HL1911] (GTX637660) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt3a antibody [HL1911] (GTX637660) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt3a antibody [HL1911] (GTX637660) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.