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Wnt3a antibody [N1C3]

Cat. No. GTX109037

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB-Ag

Reactivity

 Mouse
Package
100 μl ($399),
25 μl ($169)
See all Wnt3a products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB-Ag Assay dependent
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Predict Reactivity

Human, Rat, Zebrafish, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Wnt3a Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617265

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Wnt family member 3A

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq]

Database

Research Area

REFERENCE

Application Reference

Ciarpella F et al. STAR Protoc 2023; 4 (3) : 102413 Generation of mouse hippocampal brain organoids from primary embryonic neural stem cells.
Ciarpella F et al. iScience 2021; 24 (12) : 103438 Murine cerebral organoids develop network of functional neurons and hippocampal brain region identity.
Reactivity : Mouse
Ji Y et al. Nat Commun 2017; 8 : 15308 Adenylate kinase hCINAP determines self-renewal of colorectal cancer stem cells by facilitating LDHA phosphorylation.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169