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Wnt3a antibody

Anti-Wnt3a antibody used in Western Blot (WB). GTX128101

Cat. No. GTX128101

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all Wnt3a products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

human WNT3A-transfected 293T cells

Predict Reactivity

Mouse, Zebrafish, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.09 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Wnt3a. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885704

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Wnt family member 3A

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Wnt3a antibody used in Western Blot (WB). GTX128101

GTX128101 WB Image

Wnt3A antibody detects WNT3A protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human WNT3A-transfected 293T cells
10 % SDS-PAGE
Wnt3A antibody (GTX128101) dilution: 1:5000

REFERENCE

Application Reference

Fujino S et al. Commun Biol 2023; 6 (1) : 1191 Metastases and treatment-resistant lineages in patient-derived cancer cells of colorectal cancer.
Park M et al. Int J Mol Sci 2021; 22 (22) Human Pluripotent Stem Cell-Derived Neural Progenitor Cells Promote Retinal Ganglion Cell Survival and Axon Recovery in an Optic Nerve Compression Animal Model.
Application : WB
Reactivity : Human
Huang HC et al. Arch Dermatol Res 2019; (Epub) Lactoferrin promotes hair growth in mice and increases dermal papilla cell proliferation through Erk/Akt and Wnt signaling pathways.
Application : WB
Reactivity : Rat
Gong W et al. J Cell Biochem 2018; (Epub) Deletion of long noncoding RNA EFNA3 aggravates hypoxia-induced injury in PC-12 cells by upregulation of miR-101a.
Application : WB
Reactivity : Rat
Ng M et al. Am J Hum Genet 2017; 101 (3) : 417-427 A Genome-wide Association Study of Dupuytren Disease Reveals 17 Additional Variants Implicated in Fibrosis.
Application : IHC-P
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169