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Wnt5a antibody [6F2]

Anti-Wnt5a antibody [6F2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83127
Anti-Wnt5a antibody [6F2] used in IHC (Paraffin sections) (IHC-P). GTX83127
Anti-Wnt5a antibody [6F2] used in Western Blot (WB). GTX83127

Cat. No. GTX83127

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6F2

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, ELISA

Reactivity

 Human, Rat
Package
100 μl ($399)
See all Wnt5a products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
IHC-Fr Assay dependent
ELISA 1/10000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of WNT5A expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Wnt family member 5A , hWNT5A

Cellular Localization

Secreted

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES

Anti-Wnt5a antibody [6F2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83127

GTX83127 ICC/IF Image

ICC/IF analysis of PC12 cells using GTX83127 WNT5A antibody [6F2].
Green : WNT5A
Blue: DRAQ5 fluorescent DNA dye

Anti-Wnt5a antibody [6F2] used in IHC (Paraffin sections) (IHC-P). GTX83127

GTX83127 IHC-P Image

IHC-P analysis of human lung cancer (A), thyroid cancer (B), lymph node (C) and brain (D) using GTX83127 WNT5A antibody [6F2].

Anti-Wnt5a antibody [6F2] used in Western Blot (WB). GTX83127

GTX83127 WB Image

WB analysis of HEK293 (1) and WNT5A-hIgGFc transfected HEK293 cell lysate (2) using GTX83127 WNT5A antibody [6F2].

REFERENCE

Clone Reference

Maekawa T et al. J Dent Res 2017; 96 (5):571–577 Differential Expression and Roles of Secreted Frizzled-Related Protein 5 and the Wingless Homolog Wnt5a in Periodontitis
Application : IHC-Fr
Reactivity : Human
Submit a Reference

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399