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Wnt5a antibody [6F2]

Anti-Wnt5a antibody [6F2] used in IHC (Paraffin sections) (IHC-P). GTX83127
Anti-Wnt5a antibody [6F2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83127
Anti-Wnt5a antibody [6F2] used in Western Blot (WB). GTX83127

Cat No. GTX83127

Host

Mouse

Clonality

Monoclonal

Clone Name

6F2

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, ELISA

Reactivity

Human, Rat
Package
100 μl ($349)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites, 0.03% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of WNT5A expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Wnt family member 5A , hWNT5A

Cellular Localization

Secreted

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES

Anti-Wnt5a antibody [6F2] used in IHC (Paraffin sections) (IHC-P). GTX83127

GTX83127 IHC-P Image

IHC-P analysis of human lung cancer (A), thyroid cancer (B), lymph node (C) and brain (D) using GTX83127 WNT5A antibody [6F2].

Anti-Wnt5a antibody [6F2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83127

GTX83127 ICC/IF Image

ICC/IF analysis of PC12 cells using GTX83127 WNT5A antibody [6F2].
Green : WNT5A
Blue: DRAQ5 fluorescent DNA dye

Anti-Wnt5a antibody [6F2] used in Western Blot (WB). GTX83127

GTX83127 WB Image

WB analysis of HEK293 (1) and WNT5A-hIgGFc transfected HEK293 cell lysate (2) using GTX83127 WNT5A antibody [6F2].

REFERENCE

There are currently no references for Wnt5a antibody [6F2] (GTX83127). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Wnt5a antibody [6F2] (GTX83127). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf