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Wnt5a antibody [JU30-30]

Anti-Wnt5a antibody [JU30-30] used in Western Blot (WB). GTX01148
Anti-Wnt5a antibody [JU30-30] used in IHC (Paraffin sections) (IHC-P). GTX01148
Anti-Wnt5a antibody [JU30-30] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01148
Anti-Wnt5a antibody [JU30-30] used in Flow cytometry (FACS). GTX01148

Cat No. GTX01148

Host

Rabbit

Clonality

Monoclonal

Clone Name

JU30-30

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50
IHC-P 1:50-1:200
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Wnt family member 5A , WNT5A , hWNT5A

Cellular Localization

Secreted,Extracellular space,Extracellular matrix

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES

Anti-Wnt5a antibody [JU30-30] used in Western Blot (WB). GTX01148

GTX01148 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt5a antibody [JU30-30] (GTX01148) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Wnt5a antibody [JU30-30] used in IHC (Paraffin sections) (IHC-P). GTX01148

GTX01148 IHC-P Image

IHC-P analysis of human lung cancer tissue section using GTX01148 Wnt5a antibody [JU30-30].

Anti-Wnt5a antibody [JU30-30] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01148

GTX01148 ICC/IF Image

ICC/IF analysis of PFA-fixed PC-3M cells using GTX01148 Wnt5a antibody [JU30-30].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-Wnt5a antibody [JU30-30] used in Flow cytometry (FACS). GTX01148

GTX01148 FACS Image

FACS analysis of SH-SY-5Y cells using GTX01148 Wnt5a antibody [JU30-30].
Red : primary antibody
Black : unlabelled control
Dilution : 1:50

REFERENCE

There are currently no references for Wnt5a antibody [JU30-30] (GTX01148). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Wnt5a antibody [JU30-30] (GTX01148). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Sodium Azide.pdf