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Wnt5a antibody

Anti-Wnt5a antibody used in IHC (Paraffin sections) (IHC-P). GTX100618
Anti-Wnt5a antibody used in Western Blot (WB). GTX100618

Cat. No. GTX100618

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Wnt5a products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

WNT5A transfected 293T

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Dog, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human WNT5A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11174429

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Wnt family member 5A , hWNT5A

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Wnt5a antibody used in IHC (Paraffin sections) (IHC-P). GTX100618

GTX100618 IHC-P Image

Wnt5a antibody detects secreted Wnt5a protein by immunohistochemical analysis.
Sample: Paraffin-embedded human glioblastoma.
Wnt5a stained by Wnt5a antibody (GTX100618) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Wnt5a antibody used in Western Blot (WB). GTX100618

GTX100618 WB Image

Sample (30 ug of whole cell lysate)
A: Non-transfected 293T lysates
B: WNT5A transfected 293T lysates
10% SDS PAGE
GTX100618 diluted at 1:5000

REFERENCE

Application Reference

Shimamoto K et al. Carcinogenesis 2020; (Epub) GLIS1, a novel hypoxia-inducible transcription factor, promotes breast cancer cell motility via activation of WNT5A.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169