Wnt5a antibody detects secreted Wnt5a protein by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
Wnt5a stained by Wnt5a antibody (GTX111187) diluted at 1:500.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Wnt5a antibody detects secreted Wnt5a protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
Wnt5a stained by Wnt5a antibody (GTX111187) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with WNT5A antibody (GTX111187) diluted at 1:500.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt5a antibody (GTX111187) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt5a antibody (GTX111187) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.