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Wnt5b antibody, C-term

KO/KD
Anti-Wnt5b antibody, C-term used in Western Blot (WB). GTX47174
Anti-Wnt5b antibody, C-term used in Western Blot (WB). GTX47174

Cat. No. GTX47174

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μg ($399)
See all Wnt5b products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

HepG2

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a C-terminal region of Human WNT5B

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_11177279

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Wnt family member 5B

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Wnt5b antibody, C-term used in Western Blot (WB). GTX47174

GTX47174 WB Image

WB analysis of HepG2 cells using GTX47174 WNT5B antibody at 1.25μg/ml.
Lane A : marker
Lane B : HepG2 cells

Anti-Wnt5b antibody, C-term used in Western Blot (WB). GTX47174

GTX47174 WB Image

KO/KD

The data was published in the journal Sci Rep in 2019. PMID: 30914750

REFERENCE

Application Reference

Nava M et al. Sci Rep 2019; 9 (1) : 5141 Utilization of NGS technologies to investigate transcriptomic and epigenomic mechanisms in trastuzumab resistance.
Application : WB
Reactivity : Human
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399