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Wnt7a antibody

Anti-Wnt7a antibody used in Western Blot (WB). GTX105680
Anti-Wnt7a antibody used in IHC (Paraffin sections) (IHC-P). GTX105680
Anti-Wnt7a antibody used in IHC (Paraffin sections) (IHC-P). GTX105680

Cat No. GTX105680

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

WNT7A-transfected 293T

Predict Reactivity

Rat, Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human WNT7A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Wnt family member 7A

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

This gene is a member of the WNT gene family, which consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is involved in the development of the anterior-posterior axis in the female reproductive tract, and also plays a critical role in uterine smooth muscle pattering and maintenance of adult uterine function. Mutations in this gene are associated with Fuhrmann and Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndromes. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Wnt7a antibody used in Western Blot (WB). GTX105680

GTX105680 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with WNT7A antibody (GTX105680) diluted at 1:5000.

Anti-Wnt7a antibody used in IHC (Paraffin sections) (IHC-P). GTX105680

GTX105680 IHC-P Image

WNT7A antibody detects WNT7A protein at secreted on mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
WNT7A antibody (GTX105680) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Wnt7a antibody used in IHC (Paraffin sections) (IHC-P). GTX105680

GTX105680 IHC-P Image

WNT7A antibody detects WNT7A protein at secreted on mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
WNT7A antibody (GTX105680) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169