Wnt9a antibody [HL2422]

Wnt9a antibody [HL2422] detects Wnt9a protein by immunofluorescent analysis.
Sample: T-47D cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Wnt9a stained by Wnt9a antibody [HL2422] (GTX638644) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt9a antibody [HL2422] (GTX638644) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt9a antibody [HL2422] (GTX638644) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt9a antibody [HL2422] (GTX638644) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Wnt9a antibody [HL2422] detects secreted Wnt9a protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
Wnt9a stained by Wnt9a antibody [HL2422] (GTX638644) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Wnt9a antibody [HL2422] detects secreted Wnt9a protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
Wnt9a stained by Wnt9a antibody [HL2422] (GTX638644) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min