APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Human, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.38 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human WNT9A. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10620201
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Wnt family member 9A , WNT14
Cellular Localization
Secreted , extracellular space , extracellular matrix
Background
The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is expressed in gastric cancer cell lines. The protein encoded by this gene shows 75% amino acid identity to chicken Wnt14, which has been shown to play a central role in initiating synovial joint formation in the chick limb. This gene is clustered with another family member, WNT3A, in the chromosome 1q42 region. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-Wnt9a antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX111855](/templates/default/js/lightbox/images/loading.gif "Anti-Wnt9a antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX111855")
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GTX111855 IHC-P Image
Wnt9a antibody [N1N2], N-term detects Wnt9a protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse lung.Wnt9a stained by Wnt9a antibody [N1N2], N-term (GTX111855) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX111855 WB Image
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt9a antibody [N1N2], N-term (GTX111855) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX111855 WB Image
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt9a antibody [N1N2], N-term (GTX111855) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX111855 IHC-P Image
Wnt9a antibody [N1N2], N-term detects Wnt9a protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat heart.Wnt9a stained by Wnt9a antibody [N1N2], N-term (GTX111855) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for Wnt9a antibody [N1N2], N-term (GTX111855). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Wnt9a antibody [N1N2], N-term (GTX111855). Be the first to share your experience with this product.
![Anti-Wnt9a antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX111855](/upload/website/prouct_img/small/GTX111855/GTX111855_40121_20200703_IHC-P_M_w_23060500_941.webp "Anti-Wnt9a antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX111855")
![Anti-Wnt9a antibody [N1N2], N-term used in Western Blot (WB). GTX111855](/upload/website/prouct_img/small/GTX111855/GTX111855_40121_20200522_WB_M_lung_w_23060500_817.webp "Anti-Wnt9a antibody [N1N2], N-term used in Western Blot (WB). GTX111855")
![Anti-Wnt9a antibody [N1N2], N-term used in Western Blot (WB). GTX111855](/upload/website/prouct_img/small/GTX111855/GTX111855_40121_20200522_WB_R_heart_w_23060500_986.webp "Anti-Wnt9a antibody [N1N2], N-term used in Western Blot (WB). GTX111855")
![Anti-Wnt9a antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX111855](/upload/website/prouct_img/small/GTX111855/GTX111855_40121_20200703_IHC-P_R_w_23060500_691.webp "Anti-Wnt9a antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX111855")
![Anti-Wnt9a antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX111855](/upload/website/prouct_img/normal/GTX111855/GTX111855_40121_20200703_IHC-P_M_w_23060500_941.webp "Anti-Wnt9a antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX111855")
![Anti-Wnt9a antibody [N1N2], N-term used in Western Blot (WB). GTX111855](/upload/website/prouct_img/normal/GTX111855/GTX111855_40121_20200522_WB_M_lung_w_23060500_817.webp "Anti-Wnt9a antibody [N1N2], N-term used in Western Blot (WB). GTX111855")
![Anti-Wnt9a antibody [N1N2], N-term used in Western Blot (WB). GTX111855](/upload/website/prouct_img/normal/GTX111855/GTX111855_40121_20200522_WB_R_heart_w_23060500_986.webp "Anti-Wnt9a antibody [N1N2], N-term used in Western Blot (WB). GTX111855")
![Anti-Wnt9a antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX111855](/upload/website/prouct_img/normal/GTX111855/GTX111855_40121_20200703_IHC-P_R_w_23060500_691.webp "Anti-Wnt9a antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX111855")