APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 3-6μg/ml |
|
Note :
IHC-P
Human testis shows polar cytoplasm staining in primary spermatocytes.
|
Not tested in other applications.
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89612-PEP).
Product Note
This antibody is expected to recognise isoform d (NP_597673.1) only.
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence CGFGFRRQGEDNT, from the C Terminus of the protein sequence according to NP_597673.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
X Antigen Family Member 1B , Ct12.1 , Ct12.1C , Ct12.1D , Ct12.1E , Ct12.1B , Ctp9 , Gaged2 , Xage-1 , Xage1 , Xage1C , Xage1D , Xage1E , Xage1B
Background
This gene is a member of the XAGE subfamily, which belongs to the GAGE family. The GAGE genes are expressed in a variety of tumors and in some fetal and reproductive tissues. This gene is strongly expressed in Ewing's sarcoma, alveolar rhabdomyosarcoma and normal testis. The protein encoded by this gene contains a nuclear localization signal and shares a sequence similarity with other GAGE/PAGE proteins. Because of the expression pattern and the sequence similarity, this protein also belongs to a family of CT (cancer-testis) antigens. Alternative splicing of this gene, in addition to alternative transcription start sites, results in multiple transcript variants. [provided by RefSeq, Jan 2010]
DATA IMAGES
|
GTX89612 IHC-P Image
IHC-P analysis of human testis using GTX89612 XAGE1 (Isoform 1d) / GAGED2 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml
|
REFERENCE
There are currently no references for XAGE1 (Isoform 1d) / GAGED2 antibody, C-term (GTX89612). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for XAGE1 (Isoform 1d) / GAGED2 antibody, C-term (GTX89612). Be the first to share your experience with this product.
