APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-3μg/ml |
| 5μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88727-PEP).
Product Note
This antibody is expected to recognise isoform XBP1(S) (NP_001073007.1) only.
Predict Reactivity
Mouse, Rat, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-NHSWEDTFANELFPQ, from the internal region of the protein sequence according to NP_001073007.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
X-box binding protein 1 , TREB-5 , TREB5 , XBP-1 , XBP2
Cellular Localization
Endoplasmic reticulum , Cytoplasm , Endoplasmic reticulum membrane , Membrane , Nucleus
Background
This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX88727 IHC-P Image
IHC-P analysis of human breast using GTX88727 XBP1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml
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GTX88727 WB Image
WB analysis of HeLa nuclear lysate using GTX88727 XBP1 antibody, Internal.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer
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REFERENCE
There are currently no references for XBP1 antibody, Internal (GTX88727). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for XBP1 antibody, Internal (GTX88727). Be the first to share your experience with this product.
