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XBP1 antibody [N3C3]

Anti-XBP1 antibody [N3C3] used in Western Blot (WB). GTX102229
Anti-XBP1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102229
Anti-XBP1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX102229
Anti-XBP1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX102229
Anti-XBP1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX102229

Cat No. GTX102229

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , HepG2 (3 μM Thapsigargin treatment for 24 hr)

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human XBP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

X-box binding protein 1 , TREB-5 , TREB5 , XBP-1 , XBP2

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-XBP1 antibody [N3C3] used in Western Blot (WB). GTX102229

GTX102229 WB Image

Untreated (–) and treated (+) HepG2 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with XBP1 antibody [N3C3] (GTX102229) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-XBP1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102229

GTX102229 ICC/IF Image

XBP1 antibody [N3C3] detects XBP1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: XBP1 protein stained by XBP1 antibody [N3C3] (GTX102229) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, stained by () diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-XBP1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX102229

GTX102229 IHC-P Image

XBP1 antibody [N3C3] detects XBP1 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
XBP1 antibody [N3C3] (GTX102229) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-XBP1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX102229

GTX102229 IHC-P Image

XBP1 antibody [N3C3] detects XBP1 protein at nucleus in rat ovary by immunohistochemical analysis.
Sample: Paraffin-embedded rat ovary.
XBP1 antibody [N3C3] (GTX102229) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-XBP1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX102229

GTX102229 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using XBP1(GTX102229) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169