APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5 - 2 μg/mL |
| 10 μg/mL |
| 5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Hep G2 Cell Slide(GTX25530) , HepG2 Cell Lysate(GTX27900) , Rat Liver Tissue Lysate(GTX27943)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
XBP-1 antibody was raised against an 18 amino acid synthetic peptide from near the amino terminus of human XBP-1.The immunogen is located within amino acids 40 - 90 of XBP-1.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
X-box binding protein 1 , TREB-5 , TREB5 , XBP-1 , XBP2
Cellular Localization
Endoplasmic reticulum,Cytoplasm,Endoplasmic reticulum membrane,Membrane,Nucleus
Background
This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shu
Database
Research Area
DATA IMAGES
|
GTX31293 ICC/IF Image
ICC/IF analysis of HepG2 cells using GTX31293 XBP1 antibody.
Working concentration : 20 μg/ml
|
|
|
GTX31293 IHC-P Image
IHC-P analysis of human liver tissue using GTX31293 XBP1 antibody.
Working concentration : 20 μg/ml
|
|
|
GTX31293 IHC-P Image
IHC-P analysis of rat liver tissue using GTX31293 XBP1 antibody.
Dilution : 5 μg/ml
|
|
|
GTX31293 WB Image
WB analysis of HepG2 cell lysate using GTX31293 XBP1 antibody in (A) the absence and (B) the presence of blocking peptide.
Dilution : 1 μg/ml
|
|
|
GTX31293 IHC-P Image
IHC-P analysis of 4% paraformaldehyde-fixed human pancreas tissue using GTX31293 XBP1 antibody.
Dilution : 20 μg/ml
Green : Primary antibody
Blue : DAPI
|
|
|
GTX31293 IHC-P Image
IHC-P analysis of formaldehyde-fixed rat liver tissue using GTX31293 XBP1 antibody.
Dilution : 5 μg/ml
Antigen retrieval : Heat mediation with a citrate buffer (pH6)
|
|
|
GTX31293 WB Image
WB analysis of 100 ng of human XBP-1 recombinant protein lysate using GTX31293 XBP1 antibody.
Dilution : 0.5 μg/mL (Lane 1) / 1 μg/mL (Lane 2) / 2 μg/mL (Lane 3)
|
|
|
GTX31293 IHC-P Image
IHC-P analysis of formaldehyde-fixed mouse spleen tissue using GTX31293 XBP1 antibody.
Dilution : 2 μg/ml
Antigen retrieval : Heat mediation with a citrate buffer (pH6)
|
|
|
GTX31293 WB Image
WB analysis of HepG2 cell lysate using GTX31293 XBP1 antibody.
Dilution : 1 μg/mL (Lane 1) / 2 μg/mL (Lane 2)
Loading : 15 μg
|
|
|
GTX31293 IHC-P Image
IHC-P analysis of 4% paraformaldehyde-fixed mouse pancreas tissue using GTX31293 XBP1 antibody.
Dilution : 20 μg/ml
Green : Primary antibody
Blue : DAPI
|
|
|
GTX31293 WB Image
WB analysis of A549 cell lysate using GTX31293 XBP1 antibody.
Dilution : 0.25 μg/mL (Lane 1) / 0.5 μg/mL (Lane 2) / 1 μg/mL (Lane 3)
Loading : 15 μg
|
|
|
GTX31293 WB Image
WB analysis of rat liver tissue lysate using GTX31293 XBP1 antibody in the present and absent of blocking peptide.
Dilution : 1 μg/mL
Loading : 15 μg
|
REFERENCE
There are currently no references for XBP1 antibody (GTX31293). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for XBP1 antibody (GTX31293). Be the first to share your experience with this product.
