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XBP1 antibody

Anti-XBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31293
Anti-XBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31293
Anti-XBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31293
Anti-XBP1 antibody used in Western Blot (WB). GTX31293
Anti-XBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31293
Anti-XBP1 antibody used in Western Blot (WB). GTX31293
Anti-XBP1 antibody used in Western Blot (WB). GTX31293
Anti-XBP1 antibody used in Western Blot (WB). GTX31293
Anti-XBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31293
Anti-XBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31293
Anti-XBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31293
Anti-XBP1 antibody used in Western Blot (WB). GTX31293

Cat No. GTX31293

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 2 μg/mL
ICC/IF 10 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

Hep G2 Cell Slide(GTX25530) , HepG2 Cell Lysate(GTX27900) , Rat Liver Tissue Lysate(GTX27943)

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

XBP-1 antibody was raised against an 18 amino acid synthetic peptide from near the amino terminus of human XBP-1.The immunogen is located within amino acids 40 - 90 of XBP-1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

X-box binding protein 1 , TREB-5 , TREB5 , XBP-1 , XBP2

Cellular Localization

Endoplasmic reticulum,Cytoplasm,Endoplasmic reticulum membrane,Membrane,Nucleus

Background

This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shu

Database

Research Area

DATA IMAGES

Anti-XBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31293

GTX31293 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX31293 XBP1 antibody.
Working concentration : 20 μg/ml

Anti-XBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31293

GTX31293 IHC-P Image

IHC-P analysis of human liver tissue using GTX31293 XBP1 antibody.
Working concentration : 20 μg/ml

Anti-XBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31293

GTX31293 IHC-P Image

IHC-P analysis of rat liver tissue using GTX31293 XBP1 antibody.
Dilution : 5 μg/ml

Anti-XBP1 antibody used in Western Blot (WB). GTX31293

GTX31293 WB Image

WB analysis of HepG2 cell lysate using GTX31293 XBP1 antibody in (A) the absence and (B) the presence of blocking peptide.
Dilution : 1 μg/ml

Anti-XBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31293

GTX31293 IHC-P Image

IHC-P analysis of 4% paraformaldehyde-fixed human pancreas tissue using GTX31293 XBP1 antibody.
Dilution : 20 μg/ml
Green : Primary antibody
Blue : DAPI

Anti-XBP1 antibody used in Western Blot (WB). GTX31293

GTX31293 WB Image

WB analysis of A549 cell lysate using GTX31293 XBP1 antibody.
Dilution : 0.25 μg/mL (Lane 1) / 0.5 μg/mL (Lane 2) / 1 μg/mL (Lane 3)
Loading : 15 μg

Anti-XBP1 antibody used in Western Blot (WB). GTX31293

GTX31293 WB Image

WB analysis of 100 ng of human XBP-1 recombinant protein lysate using GTX31293 XBP1 antibody.
Dilution : 0.5 μg/mL (Lane 1) / 1 μg/mL (Lane 2) / 2 μg/mL (Lane 3)

Anti-XBP1 antibody used in Western Blot (WB). GTX31293

GTX31293 WB Image

WB analysis of rat liver tissue lysate using GTX31293 XBP1 antibody in the present and absent of blocking peptide.
Dilution : 1 μg/mL
Loading : 15 μg

Anti-XBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31293

GTX31293 IHC-P Image

IHC-P analysis of formaldehyde-fixed mouse spleen tissue using GTX31293 XBP1 antibody.
Dilution : 2 μg/ml
Antigen retrieval : Heat mediation with a citrate buffer (pH6)

Anti-XBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31293

GTX31293 IHC-P Image

IHC-P analysis of formaldehyde-fixed rat liver tissue using GTX31293 XBP1 antibody.
Dilution : 5 μg/ml
Antigen retrieval : Heat mediation with a citrate buffer (pH6)

Anti-XBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31293

GTX31293 IHC-P Image

IHC-P analysis of 4% paraformaldehyde-fixed mouse pancreas tissue using GTX31293 XBP1 antibody.
Dilution : 20 μg/ml
Green : Primary antibody
Blue : DAPI

Anti-XBP1 antibody used in Western Blot (WB). GTX31293

GTX31293 WB Image

WB analysis of HepG2 cell lysate using GTX31293 XBP1 antibody.
Dilution : 1 μg/mL (Lane 1) / 2 μg/mL (Lane 2)
Loading : 15 μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339