APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa , 293T , A431 , H1299 , NIH-3T3
Predict Reactivity
Rat, Rabbit, Bovine, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.78 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human XIAP. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1952592
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
X-linked inhibitor of apoptosis , API3 , BIRC4 , IAP-3 , ILP1 , MIHA , XLP2 , hIAP-3 , hIAP3
Background
The protein encoded by this gene is a member of a family of proteins which inhibit apoptosis through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2. This protein inhibits apoptosis induced by menadione, a potent inducer of free radicals, and ICE. It also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-XIAP antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100496](/templates/default/js/lightbox/images/loading.gif "Anti-XIAP antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100496")
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GTX100496 IHC-P Image
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using BIRC4(GTX100496) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX100496 WB Image
Wild-type (WT) and XIAP knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with XIAP antibody [C1C3] (GTX100496) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100496 WB Image
Sample (30 μg of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
GTX100496 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100496 WB Image
XIAP antibody [C1C3] detects XIAP protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg H1299 whole cell lysate/extract
7.5% SDS-PAGE
XIAP antibody [C1C3] (GTX100496) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100496 IHC-P Image
XIAP antibody [C1C3] detects XIAP protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
XIAP antibody [C1C3] (GTX100496) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX100496 ICC/IF Image
XIAP antibody [C1C3] detects XIAP protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: XIAP protein stained by XIAP antibody [C1C3] (GTX100496) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX100496 IHC-P Image
XIAP antibody [C1C3] detects XIAP protein at cytoplasm on mouse spleen by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
XIAP antibody [C1C3] (GTX100496) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
There are currently no references for XIAP antibody [C1C3] (GTX100496). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for XIAP antibody [C1C3] (GTX100496). Be the first to share your experience with this product.
![Anti-XIAP antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100496](/upload/website/prouct_img/small/GTX100496/GTX100496_IHC_w_23060100_734.webp "Anti-XIAP antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100496")
![Anti-XIAP antibody [C1C3] used in Western Blot (WB). GTX100496](/upload/website/prouct_img/small/GTX100496/GTX100496_39918_20170330_WB_KO_watermark_w_23060100_589.webp "Anti-XIAP antibody [C1C3] used in Western Blot (WB). GTX100496")
![Anti-XIAP antibody [C1C3] used in Western Blot (WB). GTX100496](/upload/website/prouct_img/small/GTX100496/GTX100496_WB_M_w_23060100_345.webp "Anti-XIAP antibody [C1C3] used in Western Blot (WB). GTX100496")
![Anti-XIAP antibody [C1C3] used in Western Blot (WB). GTX100496](/upload/website/prouct_img/small/GTX100496/GTX100496_39918_WB_w_23060100_122.webp "Anti-XIAP antibody [C1C3] used in Western Blot (WB). GTX100496")
![Anti-XIAP antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100496](/upload/website/prouct_img/small/GTX100496/GTX100496_39918_20160125_IHC-P_w_23060100_944.webp "Anti-XIAP antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100496")
![Anti-XIAP antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100496](/upload/website/prouct_img/small/GTX100496/GTX100496_39918_20150506_IFA_w_23060100_528.webp "Anti-XIAP antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100496")
![Anti-XIAP antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100496](/upload/website/prouct_img/small/GTX100496/GTX100496_39918_20150206_IHC_M_w_23060100_534.webp "Anti-XIAP antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100496")
![Anti-XIAP antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100496](/upload/website/prouct_img/normal/GTX100496/GTX100496_IHC_w_23060100_734.webp "Anti-XIAP antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100496")
![Anti-XIAP antibody [C1C3] used in Western Blot (WB). GTX100496](/upload/website/prouct_img/normal/GTX100496/GTX100496_39918_20170330_WB_KO_watermark_w_23060100_589.webp "Anti-XIAP antibody [C1C3] used in Western Blot (WB). GTX100496")
![Anti-XIAP antibody [C1C3] used in Western Blot (WB). GTX100496](/upload/website/prouct_img/normal/GTX100496/GTX100496_WB_M_w_23060100_345.webp "Anti-XIAP antibody [C1C3] used in Western Blot (WB). GTX100496")
![Anti-XIAP antibody [C1C3] used in Western Blot (WB). GTX100496](/upload/website/prouct_img/normal/GTX100496/GTX100496_39918_WB_w_23060100_122.webp "Anti-XIAP antibody [C1C3] used in Western Blot (WB). GTX100496")
![Anti-XIAP antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100496](/upload/website/prouct_img/normal/GTX100496/GTX100496_39918_20160125_IHC-P_w_23060100_944.webp "Anti-XIAP antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100496")
![Anti-XIAP antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100496](/upload/website/prouct_img/normal/GTX100496/GTX100496_39918_20150506_IFA_w_23060100_528.webp "Anti-XIAP antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100496")
![Anti-XIAP antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100496](/upload/website/prouct_img/normal/GTX100496/GTX100496_39918_20150206_IHC_M_w_23060100_534.webp "Anti-XIAP antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100496")