GeneTex
United States (US)

XIAP antibody [C1C3]

KO/KD
GTX100496_IHC_19011506_915.jpg
GTX100496_WB_M_19011506_729.jpg
GTX100496_39918_20150506_IFA_19011506_403.jpg
GTX100496_39918_WB_19011506_289.jpg
GTX100496_39918_20150206_IHC_M_19011506_347.jpg
GTX100496_39918_20160125_IHC-P_19011506_357.jpg
GTX100496_39918_20170330_WB_KO_watermark_19011506_822.jpg

Cat No. GTX100496

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

HeLa , 293T , A431 , H1299 , NIH-3T3

Predict Reactivity

Rat, Rabbit, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human XIAP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

X-Linked Inhibitor Of Apoptosis,Api3,Birc4,Iap-3,Ilp1,Miha,Xlp2,Hiap-3,Hiap3,Xiap

Background

The protein encoded by this gene is a member of a family of proteins which inhibit apoptosis through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2. This protein inhibits apoptosis induced by menadione, a potent inducer of free radicals, and ICE. It also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100496_IHC_19011506_915.jpg

GTX100496 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using BIRC4(GTX100496) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100496_WB_M_19011506_729.jpg

GTX100496 WB Image

Sample (30 µg of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
GTX100496 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100496_39918_20150506_IFA_19011506_403.jpg

GTX100496 ICC/IF Image

XIAP antibody [C1C3] detects XIAP protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: XIAP protein stained by XIAP antibody [C1C3] (GTX100496) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX100496_39918_WB_19011506_289.jpg

GTX100496 WB Image

XIAP antibody [C1C3] detects XIAP protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg H1299 whole cell lysate/extract
7.5% SDS-PAGE
XIAP antibody [C1C3] (GTX100496) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100496_39918_20150206_IHC_M_19011506_347.jpg

GTX100496 IHC-P Image

XIAP antibody [C1C3] detects XIAP protein at cytoplasm on mouse spleen by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
XIAP antibody [C1C3] (GTX100496) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100496_39918_20160125_IHC-P_19011506_357.jpg

GTX100496 IHC-P Image

XIAP antibody [C1C3] detects XIAP protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
XIAP antibody [C1C3] (GTX100496) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100496_39918_20170330_WB_KO_watermark_19011506_822.jpg

GTX100496 WB Image

KO/KD

Wild-type (WT) and XIAP knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with XIAP antibody [C1C3] (GTX100496) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169