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XIAP antibody

KO/KD
Anti-XIAP antibody used in Western Blot (WB). GTX111202
Anti-XIAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111202
Anti-XIAP antibody used in Western Blot (WB). GTX111202
Anti-XIAP antibody used in IHC (Paraffin sections) (IHC-P). GTX111202

Cat. No. GTX111202

Reference 4
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all XIAP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

Mouse brain , HeLa

Predict Reactivity

Rat, Rabbit, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human XIAP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10614971

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

X-linked inhibitor of apoptosis , API3 , BIRC4 , IAP-3 , ILP1 , MIHA , XLP2 , hIAP-3 , hIAP3

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of a family of proteins which inhibit apoptosis through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2. This protein inhibits apoptosis induced by menadione, a potent inducer of free radicals, and ICE. It also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-XIAP antibody used in Western Blot (WB). GTX111202

GTX111202 WB Image

KO/KD

Wild-type (WT) and XIAP knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with XIAP antibody (GTX111202) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-XIAP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111202

GTX111202 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using XIAP(GTX111202) antibody at 1:500 dilution.

Anti-XIAP antibody used in Western Blot (WB). GTX111202

GTX111202 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX111202 diluted at 1:1000

Anti-XIAP antibody used in IHC (Paraffin sections) (IHC-P). GTX111202

GTX111202 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hs578T xenograft, using XIAP(GTX111202) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Chang YX et al. Cancers (Basel) 2020; 12 (12) Chaperonin-Containing TCP-1 Promotes Cancer Chemoresistance and Metastasis through the AKT-GSK3β-β-Catenin and XIAP-Survivin Pathways.
Application : WB
Reactivity : Human
Wagatsuma A et al. Mol Cell Biochem 2017; (Epub) Pharmacological targeting of HSP90 with 17-AAG induces apoptosis of myogenic cells through activation of the intrinsic pathway.
Application : WB
Reactivity : Mouse
Leu JS et al. Am J Respir Crit Care Med 2016; SP110b Controls Host Immunity and Susceptibility to Tuberculosis.
Application : WB
Reactivity : Human
Lin YF et al. J Clin Invest 2013; 123 (9) : 3861-75 Targeting the XIAP/caspase-7 complex selectively kills caspase-3-deficient malignancies.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

XIAP antibody Cat. No. GTX111202

Rating
( Average 3 based on 1 users reviews)
Application
Western Blot(WB)
( Average 3 based on 1 users reviews)
Anti-XIAP antibody used in Western Blot (WB). GTX111202
Date : Anonymous submitted on 25-Jun-2019
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293 and HeLa cells
Amount used : 20 μg
Blocking : 5% non-fat milk in TBST, 25°C, 0.5Hr
Primary Antibody : 1:3000, 25°C, 2Hr
Lane Description : Lane 1: HEK293 cells; Lane 2: HeLa cells
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169