Wild-type (WT) and XIAP knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with XIAP antibody (GTX111202) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded Hs578T xenograft, using XIAP(GTX111202) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX111202 diluted at 1:1000

Immunofluorescence analysis of methanol-fixed HeLa, using XIAP(GTX111202) antibody at 1:500 dilution.