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XLF antibody [1G7]

Anti-XLF antibody [1G7] used in Western Blot (WB). GTX84043
Anti-XLF antibody [1G7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84043
Anti-XLF antibody [1G7] used in Flow cytometry (FACS). GTX84043
Anti-XLF antibody [1G7] used in Flow cytometry (FACS). GTX84043

Cat. No. GTX84043

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1G7

Isotype

 IgG1

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
100 μl ($519)
See all XLF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100
FACS 1:100
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human NHEJ1 (NP_079058) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

non-homologous end joining factor 1 , XLF

Cellular Localization

Nucleus

Background

DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary.

Database

Research Area

DATA IMAGES

Anti-XLF antibody [1G7] used in Western Blot (WB). GTX84043

GTX84043 WB Image

WB analysis of HEK293T cells transfected with XLF plasmid (Right) or empty vector (Left) for 48 hrs using GTX84043 XLF antibody [1G7].
Loading : 5 ug per lane

Anti-XLF antibody [1G7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84043

GTX84043 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with XLF plasmid using GTX84043 XLF antibody [1G7].

Anti-XLF antibody [1G7] used in Flow cytometry (FACS). GTX84043

GTX84043 FACS Image

FACS analysis of HeLa cells using GTX84043 XLF antibody [1G7].
Red : Primary antibody
Blue : Negative control antibody

Anti-XLF antibody [1G7] used in Flow cytometry (FACS). GTX84043

GTX84043 FACS Image

FACS analysis of Jurkat cells using GTX84043 XLF antibody [1G7].
Red : Primary antibody
Blue : Negative control antibody

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519