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XLF antibody, C-term

Anti-XLF antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89244
Anti-XLF antibody, C-term used in Western Blot (WB). GTX89244

Cat. No. GTX89244

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all XLF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.3μg/ml
IHC-P 2.5-3.8μg/ml

Note :

IHC-P
Human Placenta shows nuclear staining in trophoblasts.
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89244-PEP).

Predict Reactivity

Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-QRPQLSKVKRKKPR, from the C Terminus of the protein sequence according to NP_079058.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

non-homologous end joining factor 1 , XLF

Cellular Localization

Nucleus

Background

Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-XLF antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89244

GTX89244 IHC-P Image

IHC-P analysis of human placenta using GTX89244 XLF antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml

Anti-XLF antibody, C-term used in Western Blot (WB). GTX89244

GTX89244 WB Image

WB analysis of human thyroid lysate using GTX89244 XLF antibody, C-term.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399