XLF antibody [N3C3]

Cat. No. GTX100092

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all XLF products

Datasheet
Document

View product citations for antibody GTX100092 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human XLF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241458

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

non-homologous end joining factor 1 , XLF

Cellular Localization

Nucleus

Background

Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. [provided by RefSeq]

Database

Gene ID: 79840 NHEJ1
UniProt: Q9H9Q4 NHEJ1

Research Area

DATA IMAGES

GTX100092 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
12% SDS PAGE
GTX100092 diluted at 1:1000

GTX100092 IHC-P Image

XLF antibody [N3C3] detects XLF protein on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded ovarian carcinoma.
XLF antibody [N3C3] (GTX100092) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100092 ICC/IF Image

XLF antibody [N3C3] detects XLF protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: XLF protein stained by XLF antibody [N3C3] (GTX100092) diluted at 1:1000.
Blue: Hoechst 33342 staining.

GTX100092 IP Image

XLF antibody [N3C3] immunoprecipitates XLF protein in IP experiments.
IP samples: Jurkat whole cell extract
A. Control with 3 μg of preimmune Rabbit IgG
B. Immunoprecipitation of XLF protein by 3 μg XLF antibody [N3C3] (GTX100092)
10 % SDS-PAGE
The immunoprecipitated XLF protein was detected by XLF antibody [N3C3] (GTX100092) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

REFERENCE

There are currently no references for XLF antibody [N3C3] (GTX100092). Be the first to share your publications with this product.

Submit a Reference

REVIEW

There are currently no reviews for XLF antibody [N3C3] (GTX100092). Be the first to share your experience with this product.

Submit a Review

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

Document
Unmodified.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty