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XLF antibody [N3C3]

Anti-XLF antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100092
Anti-XLF antibody [N3C3] used in Western Blot (WB). GTX100092
Anti-XLF antibody [N3C3] used in Immunoprecipitation (IP). GTX100092
Anti-XLF antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX100092

Cat No. GTX100092

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

293T , HeLa , HepG2 , Raji

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human XLF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

non-homologous end joining factor 1 , XLF

Cellular Localization

Nucleus

Background

Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. [provided by RefSeq]

Database

DATA IMAGES
Anti-XLF antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100092

GTX100092 ICC/IF Image

XLF antibody [N3C3] detects XLF protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: XLF protein stained by XLF antibody [N3C3] (GTX100092) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Anti-XLF antibody [N3C3] used in Western Blot (WB). GTX100092

GTX100092 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
12% SDS PAGE
GTX100092 diluted at 1:1000

Anti-XLF antibody [N3C3] used in Immunoprecipitation (IP). GTX100092

GTX100092 IP Image

XLF antibody [N3C3] immunoprecipitates XLF protein in IP experiments.
IP samples: Jurkat whole cell extract
A. Control with 3 μg of preimmune Rabbit IgG
B. Immunoprecipitation of XLF protein by 3 μg XLF antibody [N3C3] (GTX100092)
10 % SDS-PAGE
The immunoprecipitated XLF protein was detected by XLF antibody [N3C3] (GTX100092) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Anti-XLF antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX100092

GTX100092 IHC-P Image

XLF antibody [N3C3] detects XLF protein on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded ovarian carcinoma.
XLF antibody [N3C3] (GTX100092) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
There are currently no references for XLF antibody [N3C3] (GTX100092). Be the first to share your publications with this product.
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169