GeneTex
United States (US)

XLF antibody [N3C3]

GTX100092_41570_IFA_19011506_421.jpg
GTX100092_WB_19011506_638.jpg
GTX100092_41570_IP_19011506_891.jpg
GTX100092_41031_IHC_19011506_463.jpg

Cat No. GTX100092

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

293T , HeLa , HepG2 , Raji

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human XLF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ12610 antibody, XLF antibody, NHEJ1 antibody, non-homologous end-joining factor 1 antibody, protein cernunnos antibody, XRCC4-like factor antibody, nonhomologous end-joining factor 1 antibody

Cellular Localization

Nucleus

Background

Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. [provided by RefSeq]

Database

DATA IMAGES
GTX100092_41570_IFA_19011506_421.jpg

GTX100092 ICC/IF Image

XLF antibody [N3C3] detects XLF protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: XLF protein stained by XLF antibody [N3C3] (GTX100092) diluted at 1:1000.
Blue: Hoechst 33342 staining.

GTX100092_WB_19011506_638.jpg

GTX100092 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
12% SDS PAGE
GTX100092 diluted at 1:1000

GTX100092_41570_IP_19011506_891.jpg

GTX100092 IP Image

XLF antibody [N3C3] immunoprecipitates XLF protein in IP experiments.
IP samples: Jurkat whole cell extract
A. Control with 3 μg of preimmune Rabbit IgG
B. Immunoprecipitation of XLF protein by 3 μg XLF antibody [N3C3] (GTX100092)
10 % SDS-PAGE
The immunoprecipitated XLF protein was detected by XLF antibody [N3C3] (GTX100092) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX100092_41031_IHC_19011506_463.jpg

GTX100092 IHC-P Image

XLF antibody [N3C3] detects XLF protein on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded ovarian carcinoma.
XLF antibody [N3C3] (GTX100092) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169