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XPA antibody

Anti-XPA antibody used in Western Blot (WB). GTX103168
Anti-XPA antibody used in Immunoprecipitation (IP). GTX103168
Anti-XPA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103168
Anti-XPA antibody used in IHC (Paraffin sections) (IHC-P). GTX103168

Cat No. GTX103168

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:500-1:1000
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Mouse, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human XPA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

XPA, DNA damage recognition and repair factor , XP1 , XPAC

Cellular Localization

Nucleus

Background

This gene encodes a zinc finger protein involved in DNA excision repair. The encoded protein is part of the NER (nucleotide excision repair) complext which is responsible for repair of UV radiation-induced photoproducts and DNA adducts induced by chemical carcinogens. Mutations in this gene are associated with xeroderma pigmentosum complementation group A. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-XPA antibody used in Western Blot (WB). GTX103168

GTX103168 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with XPA antibody (GTX103168) diluted at 1:2000.

Anti-XPA antibody used in Immunoprecipitation (IP). GTX103168

GTX103168 IP Image

XPA antibody immunoprecipitates XPA protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of XPA protein by 2.5 μg of XPA antibody (GTX103168) 12% SDS-PAGE The immunoprecipitated XPA protein was detected by XPA antibody (GTX103168) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-XPA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103168

GTX103168 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using XPA(GTX103168) antibody at 1:200 dilution.

Anti-XPA antibody used in IHC (Paraffin sections) (IHC-P). GTX103168

GTX103168 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using XPA(GTX103168) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169