XPA antibody

Cat. No. GTX103168

Reference 9

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all XPA products

View product citations for antibody GTX103168 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:500-1:1000

Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human XPA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952594

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

XPA, DNA damage recognition and repair factor , XP1 , XPAC

Cellular Localization

Nucleus

Background

This gene encodes a zinc finger protein involved in DNA excision repair. The encoded protein is part of the NER (nucleotide excision repair) complext which is responsible for repair of UV radiation-induced photoproducts and DNA adducts induced by chemical carcinogens. Mutations in this gene are associated with xeroderma pigmentosum complementation group A. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Database

Gene ID: 7507 XPA
UniProt: P23025 XPA

Research Area

DATA IMAGES

GTX103168 IP Image

XPA antibody immunoprecipitates XPA protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of XPA protein by 2.5 μg of XPA antibody (GTX103168) 12% SDS-PAGE The immunoprecipitated XPA protein was detected by XPA antibody (GTX103168) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX103168 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with XPA antibody (GTX103168) diluted at 1:2000.

GTX103168 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using XPA(GTX103168) antibody at 1:200 dilution.

GTX103168 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using XPA(GTX103168) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min