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XPB antibody

Anti-XPB antibody used in Western Blot (WB). GTX70311
Anti-XPB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70311
Anti-XPB antibody used in Western Blot (WB). GTX70311

Cat No. GTX70311

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
50 μg ($319)

APPLICATION

Application Note

For WB: Use at a dilution of 1:500 and incubate at RT for 40 minutes. Blocking solution: 3% Milk/TBST at RT for one hour. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

89 kDa. ( Note )

Positive Control

HeLa Nuclear extract

Specificity/Sensitivity

Human XPB

PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.2, containing no preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide corresponding to the carboxy-terminus of human XPB.

Purification

Affinity purified with antigen
From polyclonal serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Ercc Excision Repair 3, Tfiih Core Complex Helicase Subunit , Btf2 , Gtf2H , Rad25 , Tfiih , Ttd2 , Xpb , Ercc3

Cellular Localization

Nuclear

Background

The human XPB DNA helicase is a subunit of the DNA repair/basal transcription factor TFIIH, and is involved in early steps of the nucleotide excision repair (NER) pathway. Two distinct clinical phenotypes, xeroderma pigmentosum associated with Cockayne's syndrome (XP/CS) and trichothiodystrophy (TTD), can be due to mutations in the XPB gene.

Database

Research Area

DATA IMAGES

Anti-XPB antibody used in Western Blot (WB). GTX70311

GTX70311 WB Image

Anti-XPB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70311

GTX70311 ICC/IF Image

XPB antibody detects XPB protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: XPB protein stained by XPB antibody (GTX70311) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Anti-XPB antibody used in Western Blot (WB). GTX70311

GTX70311 WB Image

REFERENCE

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REVIEW

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Package List Price ($)
$ 319