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XPB antibody

Anti-XPB antibody used in Western Blot (WB). GTX70311
Anti-XPB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70311
Anti-XPB antibody used in Western Blot (WB). GTX70311

Cat. No. GTX70311

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
50 μg ($399)
See all XPB products

APPLICATION

Application Note

For WB: Use at a dilution of 1:500 and incubate at RT for 40 minutes. Blocking solution: 3% Milk/TBST at RT for one hour. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

89 kDa. ( Note )

Positive Control

HeLa Nuclear extract

Product Note

Human XPB

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide corresponding to the carboxy-terminus of human XPB.

Purification

Affinity purified with antigen
From polyclonal serum

Conjugation

Unconjugated

RRID

AB_377595

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ERCC excision repair 3, TFIIH core complex helicase subunit , BTF2 , GTF2H , RAD25 , TFIIH , TTD2 , XPB

Cellular Localization

Nucleus

Background

This gene encodes an ATP-dependent DNA helicase that functions in nucleotide excision repair. The encoded protein is a subunit of basal transcription factor 2 (TFIIH) and, therefore, also functions in class II transcription. Mutations in this gene are associated with Xeroderma pigmentosum B, Cockayne's syndrome, and trichothiodystrophy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Database

Research Area

DATA IMAGES

Anti-XPB antibody used in Western Blot (WB). GTX70311

GTX70311 WB Image

Anti-XPB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70311

GTX70311 ICC/IF Image

XPB antibody detects XPB protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: XPB protein stained by XPB antibody (GTX70311) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-XPB antibody used in Western Blot (WB). GTX70311

GTX70311 WB Image

REFERENCE

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REVIEW

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Package List Price ($)
$ 399