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XPC antibody [3.26]

Anti-XPC antibody [3.26] used in Western Blot (WB). GTX70294
Anti-XPC antibody [3.26] used in Western Blot (WB). GTX70294
Anti-XPC antibody [3.26] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70294

Cat. No. GTX70294

Reference 20

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3.26

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all XPC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

106 kDa. ( Note )

Positive Control

HeLa whole cell extract

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant XPC purified from E. coli

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_373299

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

XPC complex subunit, DNA damage recognition and repair factor , RAD4 , XP3 , XPCC , p125

Cellular Localization

Nucleus , Cytoplasm

Background

The protein encoded by this gene is a key component of the XPC complex, which plays an important role in the early steps of global genome nucleotide excision repair (NER). The encoded protein is important for damage sensing and DNA binding, and shows a preference for single-stranded DNA. Mutations in this gene or some other NER components can result in Xeroderma pigmentosum, a rare autosomal recessive disorder characterized by increased sensitivity to sunlight with the development of carcinomas at an early age. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-XPC antibody [3.26] used in Western Blot (WB). GTX70294

GTX70294 WB Image

Western blot image of human XPC from HeLa whole cell lysate detected using rabbit polyclonal (lanes 1 and 2, catalog numbers GTX70308 and GTX70309, respectively) or monoclonal (lane 3, catalog number GTX70294) antibodies.

Anti-XPC antibody [3.26] used in Western Blot (WB). GTX70294

GTX70294 WB Image

XPC antibody [3.26] detects XPC protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with XPC antibody [3.26] (GTX70294) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-XPC antibody [3.26] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70294

GTX70294 ICC/IF Image

XPC antibody [3.26] detects XPC protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: XPC protein stained by XPC antibody [3.26] (GTX70294) diluted at 1:100.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

REFERENCE

Application Reference

de Semir D et al. Cancer Res 2021; (Epub) Nuclear receptor coactivator NCOA3 regulates ultraviolet radiation-induced DNA damage and melanoma susceptibility.
Wang YC et al. Cancers (Basel) 2020; 12 (8) Downregulation of the DNA Repair Gene DDB2 by Arecoline Is through p53's DNA-Binding Domain and Is Correlated with Poor Outcome of Head and Neck Cancer Patients with Betel Quid Consumption.
Application : WB
Reactivity : Human
Pavey S et al. Mol Oncol 2020; 14 (1) : 22-41 Multiple interaction nodes define the postreplication repair response to UV-induced DNA damage that is defective in melanomas and correlated with UV signature mutation load.
Application : WB
Sears CR et al. Am J Respir Cell Mol Biol 2017; (Epub) XPC Deficiency Alters Cigarette Smoke DNA Damage Cell Fate and Accelerates Emphysema Development.
Application : WB
Reactivity : Mouse
Jia L et al. J Biol Chem 2016; (Epub) Dysregulation of CUL4A and CUL4B Ubiquitin Ligases in Lung Cancer.
Application : IHC-P
Reactivity : Human
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REVIEW

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Application Reference
de Semir D et al. Cancer Res 2021; (Epub) Nuclear receptor coactivator NCOA3 regulates ultraviolet radiation-induced DNA damage and melanoma susceptibility.
Wang YC et al. Cancers (Basel) 2020; 12 (8) Downregulation of the DNA Repair Gene DDB2 by Arecoline Is through p53's DNA-Binding Domain and Is Correlated with Poor Outcome of Head and Neck Cancer Patients with Betel Quid Consumption.
Application : WB
Reactivity : Human
Pavey S et al. Mol Oncol 2020; 14 (1):22-41 Multiple interaction nodes define the postreplication repair response to UV-induced DNA damage that is defective in melanomas and correlated with UV signature mutation load.
Application : WB
Sears CR et al. Am J Respir Cell Mol Biol 2017; (Epub) XPC Deficiency Alters Cigarette Smoke DNA Damage Cell Fate and Accelerates Emphysema Development.
Application : WB
Reactivity : Mouse
Jia L et al. J Biol Chem 2016; (Epub) Dysregulation of CUL4A and CUL4B Ubiquitin Ligases in Lung Cancer.
Application : IHC-P
Reactivity : Human
Dong TK et al. Photochem Photobiol 2016; 92 (5):760-6 Deficient Nucleotide Excision Repair in Squamous Cell Carcinoma Cells.
Application : WB
Reactivity : Human
Kim H et al. Mol Cell 2014; 54 (1):107-18 Transcriptional Repressor ZBTB1 Promotes Chromatin Remodeling and Translesion DNA Synthesis.
Application : ICC/IF
Reactivity : Human
Ramamoorthy M et al. Carcinogenesis 2013; 34 (10):2218-30 The RecQ helicase RECQL5 participates in psoralen-induced interstrand cross-link repair.
Application : WB, ICC/IF
Reactivity : Human
Demetriou SK et al. Int J Cancer 2012; 131 (8):1818-27 Defective DNA repair and cell cycle arrest in cells expressing Merkel cell polyomavirus T antigen.
Application : WB
Reactivity : Human
Acu ID et al. Cancer Res 2010; 70 (8):518859 Coordination of centrosome homeostasis and DNA repair is intact in MCF-7 and disrupted in MDA-MB 231 breast cancer cells.
Application : ICC/IF
Reactivity : Human
Takedachi A et al. Mol Cell Biol 2010; 30 (11):2708-23 DDB2 complex-mediated ubiquitylation around DNA damage is oppositely regulated by XPC and Ku and contributes to the recruitment of XPA.
Application : ICC/IF
Reactivity : Human
Rezvani HR et al. Nucleic Acids Res 2010; 38 (3):797-809 Hypoxia-inducible factor-1alpha regulates the expression of nucleotide excision repair proteins in keratinocytes.
Application : WB
Reactivity : Human
de Feraudy S et al. Am J Pathol 2010; 177 (2):555-62 The DNA damage-binding protein XPC is a frequent target for inactivation in squamous cell carcinomas.
Application : IHC-P
Reactivity : Human
Yan T et al. Clin Cancer Res 2009; 15 (22):6912-20 Differential cellular responses to prolonged LDR-IR in MLH1-proficient and MLH1-deficient colorectal cancer HCT116 cells.
Application : WB
Reactivity : Human
Klein UR et al. J Cell Sci 2009; 122 (Pt 18):3312-21 SUMO-dependent regulation of centrin-2.
Application : ICC/IF
Reactivity : Human
Ferguson-Yates BE et al. Carcinogenesis 2008; 29 (1):25689 Impaired repair of cyclobutane pyrimidine dimers in human keratinocytes deficient in p53 and p63.
Application : WB
Reactivity : Human
Wang Y et al. Cancer Res 2008; 68 (10):723791 Repair of 2'-C-cyano-2'-deoxy-1-beta-D-arabino-pentofuranosylcytosine-induced DNA single-strand breaks by transcription-coupled nucleotide excision repair.
Application : WB
Reactivity : Human
Laposa RR et al. Proc Natl Acad Sci U S A 2007; 104 (4):1389-94 Increased apoptosis, p53 up-regulation, and cerebellar neuronal degeneration in repair-deficient Cockayne syndrome mice.
Application : IHC-P
Reactivity : Mouse
Ferguson BE et al. Cancer Res 2005; 65 (19):2492294 Proficient global nucleotide excision repair in human keratinocytes but not in fibroblasts deficient in p53.
Application : WB
Reactivity : Human
Clone Reference
Adimoolam S et al. Proc Natl Acad Sci U S A 2002; 99 (20):12985-90 p53 and DNA damage-inducible expression of the xeroderma pigmentosum group C gene.
SDS
PBS.pdf
Package List Price ($)
$ 399
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