GeneTex
United States (US)

XPD antibody [N2C2], Internal

GTX105357_40275_IHC_M_19011506_342.jpg
GTX105357_40793_IFA_19011506_704.jpg
GTX105357_WB_19011506_525.jpg
GTX105357_40275_IHC_19011506_918.jpg
GTX105357_40275_20180809_ICC_IF_UVC_19011506_289.jpg

Cat No. GTX105357

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Positive Control

IMR32 , U87-MG

Predict Reactivity

Rat, Bovine, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ERCC2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

COFS2 antibody, EM9 antibody, MGC102762 antibody, MGC126218 antibody, MGC126219 antibody, TTD antibody, XPD antibody, ERCC2 antibody, basic transcription factor 2 80 kDa subunit antibody, TFIIH p80 antibody, TFIIH basal transcription factor complex helicase subunit antibody, xeroderma pigmentosum complementary group D antibody, TFIIH basal transcription factor complex 80 kDa subunit antibody, TFIIH basal transcription factor complex helicase XPD subunit antibody, DNA excision repair protein ERCC-2 antibody, CXPD antibody, xeroderma pigmentosum group D-complementing protein antibody, BTF2 p80 antibody, DNA repair protein complementing XP-D cells antibody, TFIIH 80 kDa subunit antibody, "excision repair cross-complementing rodent repair deficiency, complementation group 2 antibody"

Cellular Localization

Nucleus

Background

The nucleotide excision repair pathway is a mechanism to repair damage to DNA. The protein encoded by this gene is involved in transcription-coupled nucleotide excision repair and is an integral member of the basal transcription factor BTF2/TFIIH complex. The gene product has ATP-dependent DNA helicase activity and belongs to the RAD3/XPD subfamily of helicases. Defects in this gene can result in three different disorders, the cancer-prone syndrome xeroderma pigmentosum complementation group D, trichothiodystrophy, and Cockayne syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX105357_40275_IHC_M_19011506_342.jpg

GTX105357 IHC-P Image

ERCC2 antibody [N2C2], Internal detects ERCC2 protein at cytosol and nucleus on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
ERCC2 antibody [N2C2], Internal (GTX105357) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105357_40793_IFA_19011506_704.jpg

GTX105357 ICC/IF Image

ERCC2 antibody [N2C2], Internal detects ERCC2 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ERCC2 protein stained by ERCC2 antibody [N2C2], Internal (GTX105357) diluted at 1:500.
Blue: Hoechst 33343 staining.

GTX105357_WB_19011506_525.jpg

GTX105357 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
B: U87-MG
7.5% SDS PAGE
GTX105357 diluted at 1:1000

GTX105357_40275_IHC_19011506_918.jpg

GTX105357 IHC-P Image

Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using ERCC2(GTX105357) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105357_40275_20180809_ICC_IF_UVC_19011506_289.jpg

GTX105357 ICC/IF Image

XPD antibody [N2C2], Internal detects XPD protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: XPD stained by XPD antibody [N2C2], Internal (GTX105357) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf