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XPD antibody [N2C2], Internal

Anti-XPD antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105357
Anti-XPD antibody [N2C2], Internal used in Western Blot (WB). GTX105357
Anti-XPD antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105357
Anti-XPD antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105357

Cat. No. GTX105357

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all XPD products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Positive Control

IMR32 , U87-MG

Predict Reactivity

Rat, Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human XPD. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10616680

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ERCC excision repair 2, TFIIH core complex helicase subunit , COFS2 , EM9 , TFIIH , TTD , TTD1 , XPD

Cellular Localization

Nucleus

Background

The nucleotide excision repair pathway is a mechanism to repair damage to DNA. The protein encoded by this gene is involved in transcription-coupled nucleotide excision repair and is an integral member of the basal transcription factor BTF2/TFIIH complex. The gene product has ATP-dependent DNA helicase activity and belongs to the RAD3/XPD subfamily of helicases. Defects in this gene can result in three different disorders, the cancer-prone syndrome xeroderma pigmentosum complementation group D, trichothiodystrophy, and Cockayne syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-XPD antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105357

GTX105357 IHC-P Image

ERCC2 antibody [N2C2], Internal detects ERCC2 protein at cytosol and nucleus on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
ERCC2 antibody [N2C2], Internal (GTX105357) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-XPD antibody [N2C2], Internal used in Western Blot (WB). GTX105357

GTX105357 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
B: U87-MG
7.5% SDS PAGE
GTX105357 diluted at 1:1000

Anti-XPD antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105357

GTX105357 IHC-P Image

Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using ERCC2(GTX105357) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-XPD antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105357

GTX105357 ICC/IF Image

XPD antibody [N2C2], Internal detects XPD protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: XPD stained by XPD antibody [N2C2], Internal (GTX105357) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

REFERENCE

Application Reference

A Gautam et al. Mol Cell 2023; APE1-dependent base excision repair of DNA photodimers in human cells
Yen-Yun Wang et al. Oncol Lett 2021; 21 (6) : 450 Excision repair cross-complementing group 2 upregulation is a potential predictive biomarker for oral squamous cell carcinoma recurrence.
Application : WB, IHC-P
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169